Alliance for Pandemic Preparedness

July 8, 2020

Development and Evaluation of a Duo SARS-CoV-2 RT-QPCR Assay Combining Two Assays Approved by the World Health Organization Targeting the Envelope and the RNA-Dependant RNA Polymerase (RdRp) Coding Regions

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  • Pezzi et al. evaluated a duo SARS-CoV-2 RT-qPCR assay. Compared to monoplex RT-qPCR assays, dual-target assays may be less likely to have false-negative results caused by polymorphisms or point mutations. Based on 69 nasopharyngeal samples, the dual assay performed equal to or better than mono-target assays in terms of sensitivity, specificity, linearity, and signal intensity. 

Pezzi et al. (June 25, 2020). Development and Evaluation of a Duo SARS-CoV-2 RT-QPCR Assay Combining Two Assays Approved by the World Health Organization Targeting the Envelope and the RNA-Dependant RNA Polymerase (RdRp) Coding Regions. Viruses. https://doi.org/10.3390/v12060686