Alliance for Pandemic Preparedness

February 2, 2021

Effect of RBD (Y453F) Mutation in Spike Glycoprotein of SARS-CoV-2 on Neutralizing IgG Affinity

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[Pre-print, not peer reviewed] A mutation found in farm minks infected with SARS-CoV-2 (Y435F) was predicted to lead to reduced binding to the human ACE-2 receptor, and was experimentally shown to alter the affinity of convalescent polyclonal serum to the receptor binding domain (RBD). Using a modified ELISA assay, strong affinity to recombinant non-mutated RBD was detected in the serum of 14 out of the 21 IgG samples from convalescent patients, compared to only 2 of the 21 samples for Y435F RBD. Additionally, Y435F RBD was predicted to lower affinity compared with non-mutated RBD in four neutralizing monoclonal antibodies.

Hayashi et al. (Feb 1, 2021). Effect of RBD (Y453F) Mutation in Spike Glycoprotein of SARS-CoV-2 on Neutralizing IgG Affinity. Pre-print downloaded Feb 2 from https://doi.org/10.1101/2021.01.28.21250577