Alliance for Pandemic Preparedness

February 4, 2021

Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR

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The DETECTR assay (a combination of RT-LAMP and RT-Cas12-RNP) was found to have similar sensitivity but superior specificity to detect SARS-CoV-2 when compared to qRT-PCR on 378 patient samples derived from routine testing. Since the DETECTR assay is not as susceptible to false negatives from N-gene mutations and not as resource intensive to perform, the authors suggest the assay could complement qRT-PCR to increase testing capacity and improve specificity.

Brandsma et al. (Oct 10, 2020). Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR. The Journal of Infectious Diseases. https://doi.org/10.1093/infdis/jiaa641