Alliance for Pandemic Preparedness

February 13, 2021

Comparison of Sixteen Serological SARS-CoV-2 Immunoassays in Sixteen Clinical Laboratories

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The diagnostic accuracy of 16 antibody assays was assessed with 150 samples from people with asymptomatic, mild, or moderate COVID-19. Although confidence intervals overlapped for several assays, sensitivities in descending order were: Wantai/ELISA total-Ab (97%), CUH-NOVO/in-house ELISA total-Ab (96%), Ortho/Vitros total-Ab (95%), YHLO/iFlash-IgG (94%), Ortho/Vitros-IgG (93%), Siemens/Atellica total-Ab (93%), Roche/Elecsys total-Ab (93%), Abbott/Architect-IgG (90%), Abbott/Alinity-IgG (median 88%), Diasorin/LiaisonXL-IgG (median 85%), Siemens/Vista total-Ab (81%), Euroimmun/ELISA-IgG (78%), and Snibe/Maglumi-IgG (median 78%). The proportion of seropositivity increased with time from symptom onset and with symptom severity.

Harritshøj et al. (Feb 11, 2021). Comparison of Sixteen Serological SARS-CoV-2 Immunoassays in Sixteen Clinical Laboratories. Journal of Clinical Microbiology. https://doi.org/10.1128/JCM.02596-20