March 10, 2021

Spike Mutations in SARS-CoV-2 Variants Confer Resistance to Antibody Neutralization

Category: Article Summary

Topic: Vaccines and Immunity

Keywords (Tags): immunity, variants

• [Pre-print, not peer-reviewed] A panel of 28 SARS-CoV-2 pseudoviruses bearing single or combined spike protein mutations found in the 501Y.V1, 501Y.V2, and 501Y.V3 variants tested against a panel of monoclonal antibodies (mAbs) and convalescent patient plasma collected early in the pandemic showed that the 501Y.V2 variant was the most resistant against mAbs and convalescent plasma, followed by 501Y.V3, and then 501Y.V1. This corresponded with mutations in the N-terminal domain and receptor binding domain (RBD) of the spike protein. Analysis of the RBD carrying triple K417N/E484K/N501Y mutations found in the 501Y.V2 variant bound with mAb P2C-1F11 revealed a potential molecular basis for antibody escape.

Wang et al. (Mar 9, 2021). Spike Mutations in SARS-CoV-2 Variants Confer Resistance to Antibody Neutralization. Pre-print downloaded Mar 10 from https://doi.org/10.1101/2021.03.09.434497