Alliance for Pandemic Preparedness

March 15, 2021

Specific Allelic Discrimination of N501Y and Other SARS-CoV-2 Mutations by DdPCR Detects B.1.1.7 Lineage in Washington State

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  • [Pre-print, not peer-reviewed] A research team in Washington State described detection of the first cases of the B.1.1.7 SARS-CoV-2 variant in the state using a novel droplet reverse transcriptase digital-PCR (RT-ddPCR) assay that detects four mutations associated with the B.1.1.7 lineage. All four targets were detected in two specimens, and follow-up sequencing revealed 10 mutations in the S gene and phylogenetic clustering within the B.1.1.7 lineage. The authors suggest that RT-ddPCR may support efficient surveillance of SARS-CoV-2 strains. 

Perchetti et al. (Mar 12, 2021). Specific Allelic Discrimination of N501Y and Other SARS-CoV-2 Mutations by DdPCR Detects B.1.1.7 Lineage in Washington State. Pre-print downloaded Mar 15 from https://doi.org/10.1101/2021.03.10.21253321