January 29, 2021

A Rapid and Low-Cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-Based RT-qPCR

Category: Article Summary

Topic: Testing and Treatment

Keywords (Tags): testing, variants

[Pre-print, not peer-reviewed] Abdel-Sater et al. reported the development of a rapid molecular test to identify the SARS-CoV-2 B.1.1.7 (UK) variant using a set of RT-PCR primers that were designed to confirm the deletion mutations Δ69/Δ70 in the spike and the Δ106/Δ107/Δ108 in the NSP6 gene. The large-scale screening method may help bypass the need for widespread sequencing to confirm the presence of both the B.1.1.7 variant and variants with similar deletions.

Abdel-Sater et al. (Jan 29, 2021). A Rapid and Low-Cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-Based RT-qPCR. Pre-print downloaded Jan 29 from https://doi.org/10.1101/2021.01.27.21250048