Alliance for Pandemic Preparedness

February 19, 2021

Assessment of S1, S2 and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays

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A study examining antibody kinetics of multiple immunoglobulins in patients hospitalized with acute SARS-CoV-2 infection showed that nucleocapsid protein (NCP)-specific IgA and IgG antibodies are detected earlier, while higher spike (S)1-specific IgA antibody levels occur in severely ill patients. The analysis was conducted using a microarray, eleven different commercial immunoassays (ELISA and CLIA), and one rapid test by seven manufacturers. NCP-specific IgA and IgG antibodies continuously displayed higher detection rates than S1- and S2-specific ones, though S1-specific IgG antibodies reached higher peak values. Detection rates by commercial immunoassays generally resembled those of the microarray (corresponding to the target antigen) but sensitivities and specificities varied among all tests.

Semmler et al. (Feb 18, 2021). Assessment of S1, S2 and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays. Journal of Clinical Microbiology. https://doi.org/10.1128/JCM.02890-20