June 22, 2020

Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories

Category: Article Summary

Topic: Testing and Treatment

• Etievant et al. compared the performance of five RT-PCR-based methods developed by World Health Organization referral laboratories. Three assays known as N China CDC, N1 US CDC, and RdRp (IP2, IP4) were found to be the most sensitive assays on based on SARS-CoV-2 cell culture supernatants and clinical respiratory samples. The E Charité and N2 US CDC assays had false-positive results from negative samples and negative controls.  

Etievant et al. (June 16, 2020). Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories. Journal of Clinical Medicine. https://doi.org/10.3390/jcm9061871