General Microscopy

The Quekett Microscopical Club

Founded in 1865, this club’s members include amateurs as well as professional scientists. A very fun and informative site for microscopists of all levels.

Petr's Microscopy Resources

The links for Courses are primarily European, but are kept up to date.

Education and Tutorials

Molecular Expressions (Florida State University)

This website was the brainchild of the late Michael Davidson. It contains a wealth of information about light, optics, microscopy and imaging. Many imaging companies contributed content and support. The Nikon and Olympus links are to their versions of Molecular Expressions.

Nikon Microscopy University

Olympus Microscopy Resource Center


ImageJ Listserver

Discussion group for help with ImageJ and Fiji, applications and principles of image processing. Participants range from complete beginners to image analysis experts and programmers creating Fiji and its plugins. Freely searchable but requires free subscription to post.

Fiji Forum

Forums divided into categories such as Image Analysis, Development (of Fiji) and even job opportunities in image analysis. No registration is required to post or search.


Website for Scientific Volume Imaging, the makers of Huygens software for deconvolution and 3D analysis. A wealth of information in the Tutorials and Wiki Database found under the Support menu. The Nyquist Sampling calculator is very useful.

Intelligent Imaging Innovations, Inc (“3i”)

3i integrates microscopes and components with their Slidebook software to create innovative imaging platforms.

Interactive Fluorescent Spectra Viewers

These web based tools allow you to observe overlap between fluorophore spectra, your filters and laser lines. Helpful to understand potential bleedthrough and imaging efficiency when planning an experiment. Each viewer has its own ‘personality’ and organization of dyes and filters. They all allow either selecting commercially available filters and setting your own filter properties (usually as bandwidth and center wavelength). Pick the one that suits you best.

Molecular Probes SpectraViewer

The first spectra viewer was created by Molecular Probes (now ThermoFisher). Contains most fluorophores in their inventory, and many from their competitors. Does not include dichroic mirrors.

Fluorescent Spectra Database

Incredible wealth of information in a downloadable database from the University of Arizona. Easy to figure out, or look at the very bottom of your browser window for documentation. Allows downloading dye and filter spectra. Includes dichroic mirrors.

Chroma Spectra Viewer

From a longstanding maker of filters used in microscopy. You can overlay fluorophores onto filters selected from their catalog.

Omega Optical Curvomatic –

Omega was a pioneer in producing quality filters for fluorescent microscopy. Easily to overlay selected fluorophores onto spectra from their filters.

Histology and Specimen Preparation


A searchable resource reporting primary data, publications, and commentary on publicly available antibodies which detect human protein targets. The project is a part of the Human Antibody Initiative.

Petr's Microscopy Resources

The links for Courses are primarily European, but are kept up to date.

Histologic Stains

A website devoted to conventional histologic stains, especially those used in laboratory medicine and pathology.

University of Washington

UW Biology Imaging Center

A cost center providing training and access to light microscopy.

W.M. Keck Microscopy Center

A cost center providing training and access to light microscopy.

The Vaughan Lab

Joshua Vaughan’s group builds new tools for super-resolution fluorescent microscopy. These fluorophores combined with other traditional and recently developed approaches to study selected problems in organelle morphogenesis.

Labs and Societies

Waller Lab, UC-Berkeley

Laura Waller’s group at UC-Berkeley creates innovative imaging systems using LED arrays to create complex fields that allow computing higher spatial frequencies. Their algorithms can provide greater resolution, field of view and multiple image modalities than is possible with conventional illumination. Designs and software are available.