Keck Microscopy Facility

The Keck Microscopy Facility provides training and access to state-of-the-art light microscopy instrumentation for the entire University of Washington community as a cost center. Non-UW researchers may also use the Keck Microscopy Facility. Instrumentation includes confocal, widefield, and image restoration (deconvolution) microscopes. Access to image processing and analysis computers and software is also available.

Equipment & Software List

Training of new users, consultation with regard to selecting and optimizing imaging techniques, and advice on specimen preparation are available from the Keck Center manager, Greg Martin.

Important Notices!

6-1-08 Attention Zeiss Users! The 405 nm laser has been realigned.

4-28-08 The 2-photon laser that was part of the Leica SP1 confocal has been sold and removed. If you have been using this laser to image DAPI-labeled Nuclei, you will need to switch to a different dye. I recommend ToPro-3, which can be excited with the 633 nm laser.

11-21-07 Fluorescent filterset spectra and objective lens info has been added to the Equipment List. Check it out to determine suitability for your specimens.

08-15-07 WIRELESS! Wireless internet connectivity is now available throughout the Keck Facility. See Greg for the access code.

The Keck Imaging Center was founded as part of the W.M.Keck Center for Advanced Studies in Neural Signaling. This interdisciplinary center was established in 1990 through a grant from the W.M.Keck Foundation for researchers in the Department of Physiology and Biophysics and the Department of Pharmacology at the University of Washington in Seattle, Washington to explore how nerve cells acquire, store and transmit information.You will notice that there are changes being made to the Keck Center web site. As things change, the appearance of the updated and new pages will mirror this home page.

Let me know what works and what doesn't work, the goal is to make the web site as useful as possible!

Greg. 206-685-8784, gvm@u.washington.edu

NanoTech User Facility (NTUF) at the University of Washington provides access to emerging nanoscale tools with an emphasis on the applications of nanotechnology in biology and life sciences.  NTUF houses leading-edge instruments for characterization and fabrication at the micro- and nanoscales. Imaging tools include a Leica inverted fluorescence microscope, a Renishaw inVia Confocal Raman Microscope, a two–photon ready Zeiss LSM510 laser scanning confocal microscope, an FEI field-emission SEM with energy dispersed spectrometer, a Vecco Bioscope Scanning Probe Microscope (SPM), a Veeco Dimension 3100 SPM, and a Multimode SPM. Fabrication tools include soft lithography for rapid prototyping and a Nabity system for Electron-Beam Lithography.  NTUF operates as an open, hands-on facility available to academic and industrial users nationwide.