W. M. Keck Microscopy Center

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W. M. Keck Microscopy Center Homepage

The W. M. Keck Microscopy Center provides training and access to light microscopy instrumentation for the entire University of Washington community as a service center. Non-UW researchers may also use the W. M. Keck Microscopy Center. Instrumentation includes scanning confocal and widefield microscopes. Access to image processing and analysis computers and software is also available.

Equipment & Software List

Training of new users, consultation with regard to selecting and optimizing imaging techniques, and advice on specimen preparation are available from the Keck Center manager, Nathaniel Peters.

       
Image Processing
Links and References
 
     
 

Keck News

October 2018

A color camera has just been added to the Leica DMI6000 Inverted Widefield system. The higher sensitivity monochrome fluorescence camera is still part of this system, and is the default camera.

The color camera, unlike a monochrome fluorescence camera, takes RGB color images and is suited for imaging samples like histologically stained tissue. With its motorized stage and the Navigator mosaic tiling features of the LASX software, this system can now produce large, tiled, color images at high resolution (e.g., whole brain slices, whole kidney slices, large fields of cells).

Users interested in imaging with this color camera should contact the Keck Center manager to learn how to properly operate this device.

July 2018

The Leica Inverted Widefield system at the Keck Center has just been upgraded. The microscope stand, camera, and filter cubes have all been replaced, and the Metamorph software interface has been upgraded. Soon we will transition further from Metamorph image acquisition software to the more user friendly Leica Application Suite (LAS). Any users trained on the older Leica Inverted system can contact the Keck Center manager for complimentary re-training through September 2018.

May 2018

New Keck rates have been approved are are in effect starting May 1, 2018. Please see the Prices page for updated rates.

The current version of IMARIS is up and running at the Keck Center on a new, powerful computer workstation. This state-of-the-art image analysis software can render and analyze complicated 3D and 4D confocal datasets, as well as generate animations for presentations and publications.

There will be an IMARIS training day during the 2nd or 3rd week of June (specific date available soon) includng a morning general training session (2 hours) followed by afternoon 1-hour demos with individual users and their data. Contact the Keck Manager for more information and to be put on the IMARIS training day contact list.

 

The Keck Center is provided administrative supported by the departments of Physiology & Biophysics and Pharmacology.

The Keck Imaging Center was founded as part of the W. M. Keck Center for Advanced Studies in Neural Signaling. This interdisciplinary center was established in 1990 through a grant from the W .M. Keck Foundation for researchers in the Department of Physiology and Biophysics and the Department of Pharmacology at the University of Washington in Seattle, Washington to explore how nerve cells acquire, store and transmit information.

Let me know what works and what doesn't work, the goal is to make the web site as useful as possible!
Nathaniel. 206-685-8784, ncpeters@uw.edu

The W. M. Keck Microscopy Center is located in the Health Sciences Building K-wing, Room 507. Directions to K-wing

 

Contact Nathaniel Peters at ncpeters@uw.edu for additional information. Suggestions and comments are always welcome!

© 2018 W. M. Keck Microscopy Center, University of Washington