Keck Microscopy Facility

The Keck Microscopy Facility provides training and access to light microscopy instrumentation for the entire University of Washington community as a cost center. Non-UW researchers may also use the Keck Microscopy Facility. Instrumentation includes confocal, widefield, and image restoration (deconvolution) microscopes. Access to image processing and analysis computers and software is also available.

Equipment & Software List

Training of new users, consultation with regard to selecting and optimizing imaging techniques, and advice on specimen preparation are available from the Keck Center manager, Greg Martin.

New at the Keck

4-2-2012 The Keck Rates have been updated. Note that confocal and Widefield rates are largely unchanged (training rates are slightly lower). The DeltaVision rates have been substantially reduced.

9-7-2011 New rates have been added for "long-term" imaging on the Confocal and Deltavision Live-Cell microscopes. These rates are for experiments starting after 6 pm and lasting at least 6 hours and are significantly less than the normal rates.

3-1-2011 The Keck Center rates have been raised slightly this year. All of the rates increased by $1 - $4 per hour.

2-15-2011 The Leica SP1 confocal is no longer functional or repairable after 11 years of service. SP1 Users should schedule training on one of the other Keck Facility confocal microscopes with Greg.

6-30-2010 FREE SAMPLES! Molecular Probes has kindly provided samples of their new BacMam 2.0 CellLight reagents. These reagents can be used for transfecting cells with fluorescent proteins targeted to various organelles.

The Keck Facility has the following probes:

Non-targeted GFP (transduction control).

Synaptophysin-RFP, MAP4-RFP, PM-RFP, LAMP-GFP, Golgi-RFP.

Premo Fucci, a red/green cell cycle indicator.

Premo Autophagy assay.

4-26-2010 New Filters have been installed on the DeltaVision -- see the new filter specs here.

The Keck Imaging Center was founded as part of the W.M.Keck Center for Advanced Studies in Neural Signaling. This interdisciplinary center was established in 1990 through a grant from the W.M.Keck Foundation for researchers in the Department of Physiology and Biophysics and the Department of Pharmacology at the University of Washington in Seattle, Washington to explore how nerve cells acquire, store and transmit information.You will notice that there are changes being made to the Keck Center web site. As things change, the appearance of the updated and new pages will mirror this home page.

Let me know what works and what doesn't work, the goal is to make the web site as useful as possible!

Greg. 206-685-8784, gvm@u.washington.edu