These time-lapse recordings and still images show the behaviors of NEM/forerunner cells from the late blastula period to the end of gastrulation.
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NEM/forerunner cell position through epiboly: This embryo was followed from 40% epiboly to 95% epiboly.
Protrusive activity of NEM/forerunner cells and other marginal deep cells during early gastrulation.
NEM/forerunner cells from 40-60% epiboly: Oblique view of an embryo labeled with SYTO-11.
Forerunner cells during mid-gastrulation (65-80% epiboly): Embryo is double labeled with SYTO-11 and BODIPY 564/591.
Forerunner cells during late-gastrulation (90-100% epiboly): Converging germ ring begins to overlap the forerunner cell cluster (labeled with SYTO-11).
Forerunner cells at the end of gastrulation: BODIPY 505/515 labeled embryo.