These time-lapse recordings and still images show the behaviors of NEM/forerunner cells from the late blastula period to the end of gastrulation.
The following links take you to figure descriptions or movie descriptions and movie download options.
NEM/forerunner cell position through epiboly:
This embryo was followed from 40% epiboly to 95% epiboly.
Protrusive activity of NEM/forerunner cells
and other marginal deep cells during early gastrulation.
NEM/forerunner cells from 40-60% epiboly:
Oblique view of an embryo labeled with SYTO-11.
Forerunner cells during mid-gastrulation
(65-80% epiboly): Embryo is double labeled with SYTO-11 and BODIPY 564/591.
Forerunner cells during late-gastrulation (90-100% epiboly): Converging germ ring begins to overlap
the forerunner cell cluster (labeled with SYTO-11).
Forerunner cells at the end of gastrulation:
BODIPY 505/515 labeled embryo.