How to clone 6000 ORFs

PCR primers for amplification of ORFs and re-PCRs

• Vectors: pOBD2 and pOAD

• Strains: PJ69-4a and PJ49-4alpha

Recombination Cloning Protocols



* Find more yeast protocols on the Herskowitz Lab web page.










































HHMI,
Department of

Genome Sciences
& Medicine,
Univ. of Washington

USPS Mail:
Univ. of Washington
Box 355065
Seattle, WA 98195
Phone: (206)616-4523
Fax: (206)685-9720


Parcel Mailing:
3720 15th Ave NE
Foege Building, Room
S313
Seattle, WA 98195