Yeast Protocols
- Native gel, in-gel activity assay for ADH
- Yeast transformations (link to the Gottschling lab protocols, use "high efficiency" for fragments, either for plasmids)
- Yeast Cultures (starting cultures and harvesting for native gel ADH assay or beta-gal)
- Yeast Spore Enrichment for Random Spore Analysis
- Tetrads
- Sporulating BY4743 (link to external page)
- Sporulating other strains
- Form for analysing tetrads (pdf)
- Yeast DNA miniprep (spheroplast method)
- Yeast colony PCR (cell boiling method)
- Retrieving strains from the deletion collection
- NaOH (Kushnirov) lysates for Western blots
- Hot Acid Phenol RNA Isolation
- Immunoprecipitation + Western blot (for co-IP or concentrating a sample for Western blot)