Yeast Protocols

• Native gel, in-gel activity assay for ADH
• Yeast transformations (link to the Gottschling lab protocols, use "high efficiency" for fragments, either for plasmids)
• Yeast Cultures (starting cultures and harvesting for native gel ADH assay or beta-gal) 
• Yeast Spore Enrichment for Random Spore Analysis
• Tetrads
• Sporulating BY4743 (link to external page)
• Sporulating other strains
• Form for analysing tetrads (pdf)
• Yeast DNA miniprep (spheroplast method)
• Yeast colony PCR (cell boiling method)
• Retrieving strains from the deletion collection
• NaOH (Kushnirov) lysates for Western blots
• Hot Acid Phenol RNA Isolation
• Immunoprecipitation + Western blot (for co-IP or concentrating a sample for Western blot)