We develop new tools for high resolution fluorescence microscopy and we use these tools to understand the molecular-scale organization of biological systems in their native cellular and tissue contexts. Many of our projects make use of super-resolution fluorescence microscopy methods such as expansion microscopy and STORM in order to peer beneath the ~250 nm diffraction-limited resolution of traditional fluorescence microscopy and to interrogate nanoscale features of biological specimens with rich molecular detail. On the tool development side, we are creating chemistries for fluorescent labeling and super-resolution imaging with microscopy-based assays for single-cell epigenetic profiling. On the application side, we have partnered with biologists to apply imaging tools to aging in kidney and to immune cell development. We use an interdisciplinary approach for our research that spans chemistry, biology, and optics.
