The YRC is a NIGMS Biomedical Technology Research Center based at the University of Washington in Seattle. Click to learn more about us.
Learn more about the technologies being developed by the YRC and how they are being applied to biomedical problems.
Collaborators Since 2011
Annual NIH Dollars Impacted
We are developing technology to produce biosensors based on a ligand-binding domain (LBD) that may, in principle, be applied to any target molecule. The power of this method is illustrated in this paper by development of biosensors for digoxin and progesterone.
In this work, we combine the results of protein cross-linking mass spectrometry with iterative structural modelling to determine the molecular architecture of the 10-member Dam1p protein complex in S. cerevisiae. Using this technique, we can model conformational changes resulting from binding with microtubules.
Baboo, S et al. (2021) DeGlyPHER: An Ultrasensitive Method for the Analysis of Viral Spike N-Glycoforms. Anal Chem 93 (40):13651-13657. PubMed PMID:34597027
Ganguly, A et al. (2021) Clathrin packets move in slow axonal transport and deliver functional payloads to synapses. Neuron 109 (18):2884-2901.e7. PubMed PMID:34534453
Jiang, P et al. (2021) A modified fluctuation assay reveals a natural mutator phenotype that drives mutation spectrum variation within Saccharomyces cerevisiae. Elife 10 :. PubMed PMID:34523420
Woodall, NB et al. (2021) De novo design of tyrosine and serine kinase-driven protein switches. Nat Struct Mol Biol 28 (9):762-770. PubMed PMID:34518698
Zhou, W et al. (2021) Expanding the binding specificity for RNA recognition by a PUF domain. Nat Commun 12 (1):5107. PubMed PMID:34429425