The YRC is a NIGMS Biomedical Technology Research Center based at the University of Washington in Seattle. Click to learn more about us.
Learn more about the technologies being developed by the YRC and how they are being applied to biomedical problems.
Collaborators Since 2011
Annual NIH Dollars Impacted
We are developing technology to produce biosensors based on a ligand-binding domain (LBD) that may, in principle, be applied to any target molecule. The power of this method is illustrated in this paper by development of biosensors for digoxin and progesterone.
In this work, we combine the results of protein cross-linking mass spectrometry with iterative structural modelling to determine the molecular architecture of the 10-member Dam1p protein complex in S. cerevisiae. Using this technique, we can model conformational changes resulting from binding with microtubules.
Johnson, RS et al. (2020) Assessing Protein Sequence Database Suitability Using De Novo Sequencing. Mol. Cell Proteomics 19 (1):198-208. PubMed PMID:31732549
Jaiswal, D et al. (2020) Function of the MYND Domain and C-Terminal Region in Regulating the Subcellular Localization and Catalytic Activity of the SMYD Family Lysine Methyltransferase Set5. Mol. Cell. Biol. 40 (2):. PubMed PMID:31685550
Wang, J et al. (2019) Biochemical analysis of protein arginylation. Meth. Enzymol. 626 :89-113. PubMed PMID:31606094
Lancaster, SM et al. (2019) Fitness benefits of loss of heterozygosity in Saccharomyces hybrids. Genome Res. 29 (10):1685-1692. PubMed PMID:31548357
Read, DF et al. (2019) Predicting gene expression in the human malaria parasite Plasmodium falciparum using histone modification, nucleosome positioning, and 3D localization features. PLoS Comput. Biol. 15 (9):e1007329. PubMed PMID:31509524