Cell Function Analysis Core
The Cell Function Analysis Core facilitates the research of Diabetes Research Center affiliates by offering precise real time functional analysis of intact cells and primary tissue. Many measurements are made using a flow culture system that maintains cell viability while multiple parameters are assessed non-invasively using optical or radiological detection. With the goal of systematically studying the physiologic control of cell function in health and in diabetes, assays are offered that reflect bioenergetics, metabolism, cell signaling and biosynthetic/secretory function. The Cell Function Analysis Core also provides an islet isolation service, which gives affiliates easy access to primary tissue/cells ex vivo.
Services are offered at two locations:
Cell Function Analysis Core (UW South Lake Union Campus)
- Assays for the following parameters: lactate production, glucose production and uptake, oxygen consumption, cellular NADH, CO2 production, reductive state of cytochrome C, ROS production, mitochondrial membrane potential, ATP content, calcium and potassium metabolism. These assays have been optimized for measurements in islets, retina, stem cells, macrophages, lymphocytes, adipocytes, endothelial cells, neurons, hepatocytes, muscle and brain slices.
- Determination of of protein kinase A and C activities using state-of-the-art technology based on genetically encoded fluorescence activity biosensors. In addition, microfluidic technology that permits single cell analysis of metabolic and electrophysiologic parameters.
- Isolation and culture of pancreatic islets and other primary tissue for subsequent morphological and functional characterization. Services for specific for islet studies include measurement of insulin secretion and islet insulin content.
Cell Function Analysis Core (VA Puget Sound Health Care System)
- Isolation and culture of pancreatic islets and other primary tissue for subsequent morphological and functional characterization.
- Sophisticated metabolic phenotyping of mice allowing for quantification of glucose tolerance, insulin sensitivity and β cell function.