Chargeback Rates

CRISPR SERVICES CELL LINES

Service Unit Price*
VIRUS SERVICES
Construct for lentivirus/MMLV/AAV production per construct 670.35
Construct for shRNA lentivirus Per construct 677.96
Retro/Lentivirus production; (per roller bottle; RB) 3 / 6 / 12 RB per prep 697.00/525.78/441.30
AAV production (per roller bottle; RB) 3 / 6 / 12 RB per prep 697.00/525.78/441.30
AAV aliquot for serotype testing per aliquot 131.97
CRISPR SERVICES CELL LINES
Consultation, design and testing of guide RNA Per target 867.00
Generation of CRISPR-edited knockout line Per target 812.00
Generation of CRISPR-edited point mutation line Per target 2,029.00
Generation of CRISPR-edited knock-in line Per target 2,235.00
CRISPR SERVICES (ADDITIONAL)
Synthetic guide RNA (gRNA) (approx. $300) Each At cost
Targeting gRNA and donor construct design Hour 40.05
gRNA insert and repair construct synthesis Base 1.06
Cloning gRNA insert into targeting vector Clone 415.75
Plasmid propagation (for transfection) Sample 81.55
MOUSE RELATED SERVICES
CRISPR construct production See below Contact Core Director
Lentivirus or AAV production See Below See above
Use of Transgenic Resources Program

See Transgenic Resources Program (http://uwtransgenics.org)

The Core covers up to 20% of the cost.

Variable
MOLECULAR BIOLOGY SERVICES
Quantitative real time PCR with mRNA copy number determination
TaqMan Probe (approx. $125-$200) Each At cost
Probe and Primer Design Each 59.58
Singleplex Reaction – from RNA/DNA Well 3.15/2.70
Multiplex Reaction – from RNA/DNA Well 4.41/3.95
SYBR Green – from RNA/DNA Well 2.43/2.22
Reverse Transcription of RNA into cDNA Sample 2.94
Genotyping using TaqMan probes
Genotyping DNA Isolation Sample 11.60
25 ∝l reaction Sample 2.46
12.5 ∝l reaction Sample 2.17
Nucleic Acid Prep
Total RNA/DNA Mini Sample 16.59
Total RNA/DNA Maxi Sample 72.69
mRNA Mini Sample 45.56
mRNA Maxi Sample 193.92
Quantitation of RNA/DNA by RiboGreen/PicoGreen Well 1.24
Northern/Southern Blot
Preparation Blot 211.68
Probing of Blot Probe 237.72
Other Molecular Biology Services
Cloning of cDNA into vector Construct 677.41
Bacterial Plasmid Transformation Sample 45.05
Primer Design and Synthesis Base 1.02
Sequencing Rxn 8.80
OTHER SERVICES
Training Hour 40.05
Consultation Hour 89.72
*Invoices paid with non-UW funds will be charged an additional 8% F&A rate.

Rates are subject to change