Chargeback Rates

Service

Unit

Price*

VIRUS SERVICES

Construct for lentivirus/MMLV/AAV production

per construct

$800.26

Construct for shRNA lentivirus

Per construct

$810.31

Lentivirus production; (12×15 cm plates)

12 plates

$2853.00

AAV production (per roller bottle; RB)

3 / 6 / 12 RB per prep
*3 RB minimum

$951.00/724.00/621.00

AAV aliquot for serotype testing

per aliquot

$155.05

CRISPR SERVICES CELL LINES

Consultation, design and testing of guide RNA

Per target

$819.00

Generation of CRISPR-edited knockout line

Per target

$1,488.00

Generation of CRISPR-edited point mutation line

Per target

$2,468.00

Generation of CRISPR-edited knock-in line

Per target

$3,426.00

CRISPR SERVICES (ADDITIONAL)

Synthetic guide RNA (gRNA) (approx. $300)

Each

$300.00

Targeting gRNA and donor construct design

Hour

$50.50

gRNA insert and repair construct synthesis

Base

$1.16

Cloning gRNA insert into targeting vector

Clone

$502.90

Plasmid propagation (for transfection)

Sample

$99.30

MOUSE RELATED SERVICES

CRISPR construct production

See below

Contact Core Director

Lentivirus or AAV production

See Below

See above

Use of Transgenic Resources Program

See Transgenic Resources Program (http://uwtransgenics.org)

Variable

MOLECULAR BIOLOGY SERVICES

Quantitative real time PCR with mRNA copy number determination

TaqMan Probe

Each

$150.00

Probe and Primer Design

Each

$75.11

Singleplex Reaction – from RNA/DNA

Well

$3.71/3.25

Multiplex Reaction – from RNA/DNA

Well

$5.51/4.69

SYBR Green – from RNA/DNA

Well

$2.89/2.68

Reverse Transcription of RNA into cDNA

Sample

$3.50

Genotyping using TaqMan probes

Genotyping DNA Isolation

Sample

$13.82

25 ∝l reaction

Sample

$2.92

12.5 ∝l reaction

Sample

$2.63

Nucleic Acid Prep

Total RNA/DNA Mini

Sample

$18.81

Total RNA/DNA Maxi

Sample

$86.00

mRNA Mini

Sample

$52.22

mRNA Maxi

Sample

$224.99

Quantitation of RNA/DNA by RiboGreen/PicoGreen

Well

$1.52

Northern/Southern Blot

Preparation

Blot

$256.07

Probing of Blot

Probe

$273.23

Other Molecular Biology Services

Cloning of cDNA into vector

Construct

$800.26

Bacterial Plasmid Transformation

Sample

$51.71

Primer Design and Synthesis

Base

$1.33

Whole Plasmid Sequencing

Sample

$18.45

Sequencing

Rxn

$9.63

OTHER SERVICES

Training

Hour

$50.50

Consultation

Hour

$93.20

*Invoices paid with non-UW funds will be charged an additional 8% F&A rate.