Chargeback Rates
Service | Unit | Price* |
VIRUS SERVICES | ||
Construct for lentivirus/MMLV/AAV production | per construct | 670.35 |
Construct for shRNA lentivirus | Per construct | 677.96 |
Lentivirus production; (per roller bottle; RB) | per RB equivalent | 837.00 |
AAV production (per roller bottle; RB) | 3 / 6 / 12 RB per prep *3 RB minimum |
787.00/606.14/524.00 |
AAV aliquot for serotype testing | per aliquot | 131.97 |
CRISPR SERVICES CELL LINES | ||
Consultation, design and testing of guide RNA | Per target | 867.00 |
Generation of CRISPR-edited knockout line | Per target | 812.00 |
Generation of CRISPR-edited point mutation line | Per target | 2,029.00 |
Generation of CRISPR-edited knock-in line | Per target | 2,235.00 |
CRISPR SERVICES (ADDITIONAL) | ||
Synthetic guide RNA (gRNA) (approx. $300) | Each | At cost |
Targeting gRNA and donor construct design | Hour | 40.05 |
gRNA insert and repair construct synthesis | Base | 1.06 |
Cloning gRNA insert into targeting vector | Clone | 415.75 |
Plasmid propagation (for transfection) | Sample | 81.55 |
MOUSE RELATED SERVICES | ||
CRISPR construct production | See below | Contact Core Director |
Lentivirus or AAV production | See Below | See above |
Use of Transgenic Resources Program |
See Transgenic Resources Program (http://uwtransgenics.org) |
Variable |
MOLECULAR BIOLOGY SERVICES | ||
Quantitative real time PCR with mRNA copy number determination | ||
TaqMan Probe (approx. $125-$200) | Each | At cost |
Probe and Primer Design | Each | 59.58 |
Singleplex Reaction – from RNA/DNA | Well | 3.15/2.70 |
Multiplex Reaction – from RNA/DNA | Well | 4.41/3.95 |
SYBR Green – from RNA/DNA | Well | 2.43/2.22 |
Reverse Transcription of RNA into cDNA | Sample | 2.94 |
Genotyping using TaqMan probes | ||
Genotyping DNA Isolation | Sample | 11.60 |
25 ∝l reaction | Sample | 2.46 |
12.5 ∝l reaction | Sample | 2.17 |
Nucleic Acid Prep | ||
Total RNA/DNA Mini | Sample | 16.59 |
Total RNA/DNA Maxi | Sample | 72.69 |
mRNA Mini | Sample | 45.56 |
mRNA Maxi | Sample | 193.92 |
Quantitation of RNA/DNA by RiboGreen/PicoGreen | Well | 1.24 |
Northern/Southern Blot | ||
Preparation | Blot | 211.68 |
Probing of Blot | Probe | 237.72 |
Other Molecular Biology Services | ||
Cloning of cDNA into vector | Construct | 677.41 |
Bacterial Plasmid Transformation | Sample | 45.05 |
Primer Design and Synthesis | Base | 1.02 |
Sequencing | Rxn | 8.80 |
OTHER SERVICES | ||
Training | Hour | 40.05 |
Consultation | Hour | 89.72 |
*Invoices paid with non-UW funds will be charged an additional 8% F&A rate. |
Rates are subject to change