Chargeback Rates
| Service | Unit | Price* |
| VIRUS SERVICES | ||
| Construct for lentivirus/MMLV/AAV production | per construct | 670.35 |
| Construct for shRNA lentivirus | Per construct | 677.96 |
| Lentivirus production; (per roller bottle; RB) | per RB equivalent | 837.00 |
| AAV production (per roller bottle; RB) | 3 / 6 / 12 RB per prep *3 RB minimum |
787.00/606.14/524.00 |
| AAV aliquot for serotype testing | per aliquot | 131.97 |
| CRISPR SERVICES CELL LINES | ||
| Consultation, design and testing of guide RNA | Per target | 867.00 |
| Generation of CRISPR-edited knockout line | Per target | 812.00 |
| Generation of CRISPR-edited point mutation line | Per target | 2,029.00 |
| Generation of CRISPR-edited knock-in line | Per target | 2,235.00 |
| CRISPR SERVICES (ADDITIONAL) | ||
| Synthetic guide RNA (gRNA) (approx. $300) | Each | At cost |
| Targeting gRNA and donor construct design | Hour | 40.05 |
| gRNA insert and repair construct synthesis | Base | 1.06 |
| Cloning gRNA insert into targeting vector | Clone | 415.75 |
| Plasmid propagation (for transfection) | Sample | 81.55 |
| MOUSE RELATED SERVICES | ||
| CRISPR construct production | See below | Contact Core Director |
| Lentivirus or AAV production | See Below | See above |
| Use of Transgenic Resources Program |
See Transgenic Resources Program (http://uwtransgenics.org) |
Variable |
| MOLECULAR BIOLOGY SERVICES | ||
| Quantitative real time PCR with mRNA copy number determination | ||
| TaqMan Probe (approx. $125-$200) | Each | At cost |
| Probe and Primer Design | Each | 59.58 |
| Singleplex Reaction – from RNA/DNA | Well | 3.15/2.70 |
| Multiplex Reaction – from RNA/DNA | Well | 4.41/3.95 |
| SYBR Green – from RNA/DNA | Well | 2.43/2.22 |
| Reverse Transcription of RNA into cDNA | Sample | 2.94 |
| Genotyping using TaqMan probes | ||
| Genotyping DNA Isolation | Sample | 11.60 |
| 25 ∝l reaction | Sample | 2.46 |
| 12.5 ∝l reaction | Sample | 2.17 |
| Nucleic Acid Prep | ||
| Total RNA/DNA Mini | Sample | 16.59 |
| Total RNA/DNA Maxi | Sample | 72.69 |
| mRNA Mini | Sample | 45.56 |
| mRNA Maxi | Sample | 193.92 |
| Quantitation of RNA/DNA by RiboGreen/PicoGreen | Well | 1.24 |
| Northern/Southern Blot | ||
| Preparation | Blot | 211.68 |
| Probing of Blot | Probe | 237.72 |
| Other Molecular Biology Services | ||
| Cloning of cDNA into vector | Construct | 677.41 |
| Bacterial Plasmid Transformation | Sample | 45.05 |
| Primer Design and Synthesis | Base | 1.02 |
| Sequencing | Rxn | 8.80 |
| OTHER SERVICES | ||
| Training | Hour | 40.05 |
| Consultation | Hour | 89.72 |
| *Invoices paid with non-UW funds will be charged an additional 8% F&A rate. | ||
Rates are subject to change