Chargeback Rates

Service

Unit

Price*

VIRUS SERVICES

Construct for lentivirus/MMLV/AAV production

per construct

$715.04

Construct for shRNA lentivirus

Per construct

$725.10

Lentivirus production; (12×15 cm plates)

$2853.00

AAV production (per roller bottle; RB)

3 / 6 / 12 RB per prep
*3 RB minimum

$951.00/724.00/621.00

AAV aliquot for serotype testing

per aliquot

$140.28

CRISPR SERVICES CELL LINES

Consultation, design and testing of guide RNA

Per target

$771.00

Generation of CRISPR-edited knockout line

Per target

$1,363.00

Generation of CRISPR-edited point mutation line

Per target

$2,243.00

Generation of CRISPR-edited knock-in line

Per target

$3,068.00

CRISPR SERVICES (ADDITIONAL)

Synthetic guide RNA (gRNA) (approx. $300)

Each

$300.00

Targeting gRNA and donor construct design

Hour

$43.81

gRNA insert and repair construct synthesis

Base

$1.10

Cloning gRNA insert into targeting vector

Clone

$446.09

Plasmid propagation (for transfection)

Sample

$87.94

MOUSE RELATED SERVICES

CRISPR construct production

See below

Contact Core Director

Lentivirus or AAV production

See Below

See above

Use of Transgenic Resources Program

See Transgenic Resources Program (http://uwtransgenics.org)

Variable

MOLECULAR BIOLOGY SERVICES

Quantitative real time PCR with mRNA copy number determination

TaqMan Probe

Each

$150.00

Probe and Primer Design

Each

$65.17

Singleplex Reaction – from RNA/DNA

Well

$3.35/2.90

Multiplex Reaction – from RNA/DNA

Well

$4.68/4.22

SYBR Green – from RNA/DNA

Well

$2.59/2.38

Reverse Transcription of RNA into cDNA

Sample

$3.14

Genotyping using TaqMan probes

Genotyping DNA Isolation

Sample

$12.40

25 ∝l reaction

Sample

$2.62

12.5 ∝l reaction

Sample

$2.33

Nucleic Acid Prep

Total RNA/DNA Mini

Sample

$17.39

Total RNA/DNA Maxi

Sample

$77.48

mRNA Mini

Sample

$47.96

mRNA Maxi

Sample

$205.11

Quantitation of RNA/DNA by RiboGreen/PicoGreen

Well

$1.34

Northern/Southern Blot

Preparation

Blot

$227.67

Probing of Blot

Probe

$250.51

Other Molecular Biology Services

Cloning of cDNA into vector

Construct

$715.04

Bacterial Plasmid Transformation

Sample

$47.45

Primer Design and Synthesis

Base

$1.10

Sequencing

Rxn

$8.92

OTHER SERVICES

Training

Hour

$43.81

Consultation

Hour

$90.97

*Invoices paid with non-UW funds will be charged an additional 8% F&A rate.