Services and Facilities
Metabolic Assay Services
The HSC is now able to offer assays to affiliates through the Northwest Lipid Metabolism and Diabetes Research Laboratories (NWRL) at reduced cost and with a rapid turnaround. These assays are primarily for human plasma measurements, but could be used on samples from animals, cell lysates or culture media. A list of analyses currently available and the cost per sample is provided on the chargeback schedule. In addition, the NWRL will work with affiliates to develop novel assays as needed to assist them in achieving their research objectives.
Hyperinsulinemic-euglycemic clamps can be performed with or without tracers by a specially trained registered nurse practitioner for measurement of hepatic, muscle and adipose tissue insulin sensitivity. Training in these techniques can also be provided upon request.
Hyperglycemic clamps with or without arginine and stepped hyperglycemic clamps are provided by the Human Studies Core to measure insulin secretion and β cell function.
The Human Studies Core also can provide training in or performance of oral glucose or meal tolerance tests and intravenous glucose tolerance tests.
Data from frequently sampled intravenous glucose tolerance tests (FSIGT) can be modeled using MinMod, developed by Bergman et al (Pacini G et al, Computer methods and programs in biomedicine 1986; 23:113-122). This provides measures of insulin sensitivity, insulin release and glucose disposal.
Oral glucose and meal tolerance data will be modeled using SAAM II software (Cobelli C, Foster DM, Adv Exptl Med Biol 1998; 445:79-101) based on the model developed by Cobelli et al (Dalla Man C et al, Diabetes 2005; 54:3265-3273). This model provides various measures of the β cell’s ability to respond to glucose as measures of β cell function.
Metabolic phenotyping procedures are conducted at the UW Medical Center (General Clinical Research Center) or at VA Puget Sound Health Care Center (Clinical Research Unit and in space occupied by the Diabetes Research Group).
The Human Studies Core can facilitate access and streamline the process for identifying potential study subjects through Electronic Medical Record (EMR) systems. The Human Studies Core can review VA EMR to identify potential subjects for recruitment into both observational and clinical trial research to investigators or a study team that includes a VA investigator. EMR data can be accessed for an individual medical center, a VISN, or nationally. Those wishing to recruit subjects or use data from the University of Washington system will be directed to the ITHS EMR patient recruitment service.
Consultation and Statistical Analysis
Expert assistance with the design and conduct of clinical research, subject recruitment, and statistical analysis. The Human Studies Core provides diabetes focused consultation on research design, methods, and data analysis not easily obtained from elsewhere in the UW research community. This resource takes advantage of the extensive experience of the Human Studies Core director and associate directors.
Japanese American Community Diabetes Repository
The Japanese American Community Diabetes Study repository has collected extensive information and to date has yielded over 70 scientific publications authored by not only local scientists but also investigators from around the world. It includes extensive phenotyping and stored plasma and white blood cells from a 10 year prospective evaluation of 658 Japanese Americans who resided in King County, Washington State. Measurements were conducted at baseline, 5- and 10-years and include glucose, insulin, and C-peptide concentrations for a 180-minute oral glucose tolerance test; CT-measurements of visceral and multiple subcutaneous fat depots; lipid profile; and a focused physical examination and medical history.