Quantitative and Functional Proteomics Core
The overall goals of the QFPC are to provide the powerful tools of modern mass spectrometry and complex data set analysis to Diabetes Research Center (DRC) investigators to permit structural identification and quantitation of proteins involved in diabetes and its complications.
To accomplish these goals the QFPC provides the following to DRC affiliate investigators:
- To perform mass spectrometric (MS) analyses for DRC investigators, such as quantifying target analytes and obtaining spectra for structural identification of proteins. Technologies include electrospray ionization tandem mass spectrometry (ESI-MS/MS), matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS, and TOF/TOF MS/MS.
- To provide and maintain functional MS systems for DRC studies.
- To perform collaborative research studies involving Core staff and DRC affiliates.
- To develop new MS methods for structural identification or quantification of biomolecules involved in the pathogenesis of diabetes and its complications, risk factors, or treatment.
- To provide a central facility for data storage, dissemination, and sharing.
- To provide training in principles of MS and using MS systems, including gas chromatography (GC/MS), electrospray ionization (ESI-MS/MS), and MALDI-TOF-MS/MS analysis.
- To reduce the cost of DRC research by providing a centralized MS/informatics facility at a fraction of the cost of maintaining instruments and computational support in individual investigators’ laboratories or of using commercial MS facilities.
- To provide bioinformatics support for analyzing and interpreting proteomic data sets and for integrating them with Gene Ontology, protein-protein interaction databases, and pathway analysis.
- To integrate proteomic studies with functional studies, with the long-term aim of providing an integrated, systems biology view of diabetes and diabetes-related disease processes.