Chargeback Rates

Service Unit Price*
VIRUS SERVICES
Construct for lentivirus/MMLV/AAV production per construct 360.54
Construct for shRNA lentivirus Per construct 391.69
Retro/Lentivirus production; no TFF 25 plates
50 plates
606.86
831.62
Retro/Lentivirus production; small step TFF 25 plates
50 plates
747.20
962.17
AAV production (serotypes 1-9 and DJ) 12 plates
25 plates
50 plates
699.11
945.31
1,125.34
RCR testing per virus 143.96
Construct for adenovirus production per construct 360.54
Adenovirus production per virus ~1,200
AAV aliquot for serotype testing per aliquot 97.59
CRISPR SERVICES
Targeting guide RNA (gRNA) and repair construct design Hour 36.33
gRNA insert and repair construct synthesis Base 1.02
Cloning gRNA insert into targeting vector Clone 390.43
Plasmid propagation (for transfection) Sample 75.22
MOUSE RELATED SERVICES
Targeting construct for generation of genetically engineered mice:
Targeting construct generation for transgenic or knockout mice Each 2,349.12
or at cost
CRISPR and virus constructs for generation of transgenic mice:
CRISPR construct production See Below See Below
Targeting guide RNA (gRNA) and repair construct design Hour 36.33
gRNA insert and repair construct synthesis Base 1.02
Cloning gRNA insert into targeting vector Clone 390.43
Plasmid propagation (as microinjection donor) Sample 75.22
Production of Cas9 mRNA and gRNA for mouse microinjection Sample 360.92
Lentivirus production See Above See Above
Use of Transgenic Resources Program See Transgenic Resources Program (http://uwtransgenics.org) The Core covers up to 20% of the cost. Variable
MOLECULAR BIOLOGY SERVICES
Quantitative real time PCR with mRNA copy number determination
TaqMan Probe (approx. $125-$200) Each At cost
Probe and Primer Design Each 54.04
Singleplex Reaction – from RNA/DNA Well 2.96 / 2.50
Multiplex Reaction – from RNA/DNA Well 4.15 / 3.69
SYBR Green – from RNA/DNA Well 2.26 / 2.05
Reverse Transcription of RNA into cDNA Sample 2.74
Genotyping using TaqMan probes
Genotyping DNA Isolation Sample 10.81
25 ∝l reaction Sample 2.29
12.5 ∝l reaction Sample 2.00
Nucleic Acid Prep
Total RNA/DNA Mini Sample 15.80
Total RNA/DNA Maxi Sample 67.94
mRNA Mini Sample 43.19
mRNA Maxi Sample 182.84
Quantitation of RNA/DNA by RiboGreen/PicoGreen Well 1.14
Northern/Southern Blot
Preparation Blot 148.56
Probing of Blot Probe 187.22
Other Molecular Biology Services
Cloning of cDNA into vector Construct 360.54
Bacterial Plasmid Transformation Sample 44.73
Primer Design and Synthesis Base 0.94
Sequencing Rxn 11.82
OTHER SERVICES
Training Hour 36.33
Consultation Hour 55.81
*Invoices paid with non-UW funds will be charged an additional 8% F&A rate.

 

Rates are subject to change