Chargeback Rates

Service Unit Price*
VIRUS SERVICES
Construct for lentivirus/MMLV/AAV production per construct 528.10
Construct for shRNA lentivirus Per construct 535.70
Retro/Lentivirus production; no TFF 25 plates
50 plates
759.02
1,025.44
Retro/Lentivirus production; small step TFF 25 plates
50 plates
933.77
1,189.51
AAV production (serotypes 1-9 and DJ) 12 plates
25 plates
50 plates
751.92
1,099.10
1,408.89
RCR testing per virus 184.22
AAV aliquot for serotype testing per aliquot 107.31
CRISPR SERVICES CELL LINES
Consultation, design and testing of guide RNA Per target 860.00
Generation of CRISPR-edited knockout line Per target 800.00
Generation of CRISPR-edited point mutation line Per target 2,000.00
Generation of CRISPR-edited knock-in line Per target 2,200.00
MOUSE RELATED SERVICES
CRISPR construct production See below Contact Core Director
Lentivirus or AAV production See Below See above
Use of Transgenic Resources Program See Transgenic Resources Program (http://uwtransgenics.org)

The Core covers up to 20% of the cost.

Variable
MOLECULAR BIOLOGY SERVICES
Quantitative real time PCR with mRNA copy number determination
TaqMan Probe (approx. $125-$200) Each At cost
Probe and Primer Design Each 58.22
Singleplex Reaction – from RNA/DNA Well 3.11 / 2.65
Multiplex Reaction – from RNA/DNA Well 4.35 / 3.89
SYBR Green – from RNA/DNA Well 2.39 / 2.18
Reverse Transcription of RNA into cDNA Sample 2.89
Genotyping using TaqMan probes
Genotyping DNA Isolation Sample 11.41
25 ∝l reaction Sample 2.42
12.5 ∝l reaction Sample 2.13
Nucleic Acid Prep
Total RNA/DNA Mini Sample 16.40
Total RNA/DNA Maxi Sample 71.52
mRNA Mini Sample 44.98
mRNA Maxi Sample 191.20
Quantitation of RNA/DNA by RiboGreen/PicoGreen Well 1.22
Northern/Southern Blot
Preparation Blot 164.27
Probing of Blot Probe 199.79
Other Molecular Biology Services
Cloning of cDNA into vector Construct 528.10
Bacterial Plasmid Transformation Sample 44.47
Primer Design and Synthesis Base 1.00
Sequencing Rxn 8.70
OTHER SERVICES
Training Hour 39.14
Consultation Hour 76.29
*Invoices paid with non-UW funds will be charged an additional 8% F&A rate.

Rates are subject to change