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Chlamydia Transport Medium differs from Viral Transport Medium; they are not interchangeable. Wooden swabs and calcium alginate swabs inhibit the growth of Chlamydia and should never be used to culture for Chlamydia. Dacron swabs are recommended and may be requested from Reference Laboratory Services. A nasopharyn-geal swab obtained from both sides of the posterior pharynx is the optimal method for culturing infants suspected of having infant pneumonia syndrome. For infants with conjunctivitis, swab the lower conjunctive of both eyes. Tissues can also be cultured by having either the swab or biopsy dropped into the Chlamydia Transport Medium. The optimal genital sites for chlamydia isolation are the urethra in men and the urethra and/or endocervix in women (separate swabs can be combined for culture). Rectal swabs should be collected from homosexual and bisexual males suspected of having a rectal chlamydial infection. Chlamydia specimens can be stored at 4°C for 48 hours without significant loss of titer or immediately frozen at -70°C if transportation is delayed. Avoid freezing at -4°C or -20°C. Ship to UWMC, Room NW 220.
Search "CHLC" code in the online Laboratory Test Catalog for information on Chlamydia Trachomatis Culture.
Monoclonal antibodies are currently being used in a DFA assay to detect C. trachomatis in clinical specimens. For these tests; conjunctival, endocervical, nasopharyngeal, rectal or urethral swabs should be spotted onto clean glass slides. (Note: We prefer that you make the slide for Chlamydia at the time of specimen collection. Air dry the slide completely. Flood the slide with methanol and allow to air dry.) Either culture or nucleic acid amplification assays are preferable in women during menses.
Search "CHLFA" code in the online Laboratory Test Catalog for information on Chlamydia Trachomatis Fluorescent Antibody.
Chlamydia serologies are also available. These include C. trachomatis, lymphogranuloma venereum, and C. pneumoniae (TWAR). The indirect fluorescent antibody (IFA) assays determine IgG (past or persistent infections) and IgM (acute infection) levels. Only one serum sample is required (approximately 1 mL). Acute and convalescent (four weeks) sera are recommended for C. pneumoniae analysis. When analyzing results of Chlamydia serologies, it is helpful to note the date of onset and whether or not the specimen is considered to be acute or convalescent.
Links to specific information can be found by searching these codes in the online Laboratory Test Catalog:
"CHLTWS" for IFA - C. pneumoniae - convalescent
"CHLTWP" for IFA - C. pneumoniae - paired
"CHLSS" for IFA - C. trachomatis
"CHLSP" for IFA - C. trachomatis - paired
Gen-Probe Aptima Combo 2 assay collection kits are provided for your use. Please use the large swab to cleanse the specimen site of excess mucus and then use the small swab with blue shaft to obtain the specimen. Urethral specimens should be collected at least 1 hour after last urination. Urine should be transported in a polypropylene tube. Do not submit a clean catch.
Search "CHLAD" code in the online Laboratory Test Catalog for information on Chlamydia Trachomatis (CT) Amplif Nucleic Acid Detection information.
M5 media is provided for Chlamydia pneumoniae culture upon request. Viral or Mycoplasma transport media should not be submitted. Chlamydia trachomatis media, CTM, or M4 media are acceptable but not optimal. Nasopharyngeal or BAL specimens are recommended. Swabs from spectrum corporation and those with wooden shafts or calcium alginate fibers inhibit chlamydia and should be avoided.
Search "CHLPC" code in the online Laboratory Test Catalog for information on Chlamydia Pneumoniae Culture.
Last updated: 11/27/12