CRISPR, Vector and Transgenic Mouse Core

The overall objective of the CRISPR, Vector and Transgenic Mouse Core (CVTMC) is to provide Diabetes Research Center (DRC) affiliate investigators with state-of-the-art reagents necessary to edit, monitor, or alter expression of genes and RNAs of interest in cultured cells, tissues, and animals. To accomplish this goal the CRISPR, Vector and Transgenic Mouse Core provides the following to Diabetes Research Center affiliate investigators:

• Production of CRISPR/Cas9 reagents for genome editing;
• Production of CRISPR reagents for various other applications, such as CRISPR-mediated transcriptional activation and repression;
• Production of adeno-associated viral (AAV) and lentiviral vectors for use in animals, tissues and cells;
• CRISPR/Cas9-directed gene editing in mice through a collaboration with the UW Mouse CRISPR Core, UW Mouse CRISPR Core;
• Cost-effective cryopreservation of mouse lines;
• Generation of cell lines by CRISPR/Cas9 in collaboration with the Institute for Stem Cell & Regenerative Medicine (ISCRM);
• Development of genotyping methods, genotyping and sequencing to validate edited mice and cell lines;
• Other molecular biology techniques not routinely performed in affiliate investigators’ laboratories;
• Consultation at each step;
• Training of affiliates and their trainees in the methods used by the Core.