Chargeback Rates

Service

Unit

Price*

VIRUS SERVICES

Construct for lentivirus/MMLV/AAV production

per construct

$817.40

Construct for shRNA lentivirus

Per construct

$838.90

Lentivirus production; (12×15 cm plates)

12 plates

$3,566.00

AAV production (per roller bottle; RB)

3 / 6 / 12 RB per prep
*3 RB minimum

$1,190.00/1,050.00/900.00

AAV aliquot for serotype testing

per aliquot

$156.80

CRISPR SERVICES CELL LINES

Consultation, design and testing of guide RNA

Per target

$822.90

Generation of CRISPR-edited knockout line

Per target

$1,433.00

Generation of CRISPR-edited point mutation line

Per target

$2,437.00

Generation of CRISPR-edited knock-in line

Per target

$3,537.00

CRISPR SERVICES (ADDITIONAL)

Synthetic guide RNA (gRNA) (approx. $300)

Each

$300.00

Targeting gRNA and donor construct design

Hour

$51.28

gRNA insert and repair construct synthesis

Base

$1.17

Cloning gRNA insert into targeting vector

Clone

$521.50

Plasmid propagation (for transfection)

Sample

$119.80

MOUSE RELATED SERVICES

CRISPR construct production

See below

Contact Core Director

Lentivirus or AAV production

See Below

See above

Use of Transgenic Resources Program

See Transgenic Resources Program (http://uwtransgenics.org)

Variable

MOLECULAR BIOLOGY SERVICES

Quantitative real time PCR with mRNA copy number determination

TaqMan Probe

Each

$200.00

Probe and Primer Design

Each

$76.28

Singleplex Reaction – from RNA/DNA

Well

$3.75/3.39

Multiplex Reaction – from RNA/DNA

Well

$5.21/4.85

SYBR Green – from RNA/DNA

Well

$3.14/2.93

Reverse Transcription of RNA into cDNA

Sample

$4.25

Genotyping using TaqMan probes

Genotyping DNA Isolation

Sample

$13.55

25 ∝l reaction

Sample

$3.08

12.5 ∝l reaction

Sample

$2.73

Nucleic Acid Prep

Total RNA/DNA Mini

Sample

$17.83

Total RNA/DNA Maxi

Sample

$104.90

mRNA Mini

Sample

$56.22

mRNA Maxi

Sample

$252.20

Quantitation of RNA/DNA by RiboGreen/PicoGreen

Well

$1.54

Northern/Southern Blot

Preparation

Blot

$250.40

Probing of Blot

Probe

$280.80

Other Molecular Biology Services

Cloning of cDNA into vector

Construct

$817.40

Bacterial Plasmid Transformation

Sample

$45.32

Primer Design and Synthesis

Base

$1.35

Whole Plasmid Sequencing

Sample

$18.65

Sequencing

Rxn

$9.76

OTHER SERVICES

Training

Hour

$51.28

Consultation

Hour

$93.46

*Invoices paid with non-UW funds will be charged an additional 8% F&A rate.