Alliance for Pandemic Preparedness

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Tag: wastewater

June 4, 2021

A Comparison of Four Commercially Available RNA Extraction Kits for Wastewater Surveillance of SARS-CoV-2 in a College Population

[Pre-print, not peer-reviewed] A comparison of four commercially available RNA extraction kits found that the Zymo Quick-RNA Viral kit yielded the most consistent, timely, and accurate results for detection of SARS-CoV-2 in wastewater from a college campus. Although wastewater-based surveillance has been performed as an early detection system for enteric viruses, most of these viruses…

January 26, 2021

Predictive Power of SARS-CoV-2 Wastewater Surveillance for Diverse Populations across a Large Geographical Range

[Pre-print, not peer-reviewed] Trends in the detection of SARS-CoV-2 RNA in wastewater appeared to precede trends in clinically confirmed cases in Minnesota during the summer 2020 wave. Viral RNA was detected in municipal wastewater influent continually for 20 weeks in cities ranging from 500 to 1 million people. Statewide data showed that trends in clinically…

November 24, 2020

Catching a Resurgence Increase in SARS-CoV-2 Viral RNA Identified in Wastewater 48 Hours before COVID-19 Clinical Tests and 96 Hours before Hospitalizations

[Pre-print, not peer reviewed] Surveillance for SARS-CoV-2 at wastewater facilities in Ottawa identified resurgence of positive cases and hospitalizations in late July 2020 with a 48-hour and 96- hour lead time, respectively. When daily percent positivity was being reported below 1%, increases of >400% in normalized SARS-CoV-2 RNA signal in wastewater were identified 48 hours…

November 9, 2020

COVID-19 Wastewater Epidemiology A Model to Estimate Infected Populations

[Preprint, not peer-reviewed] A susceptible-exposed-infectious-removed (SEIR) model based on composite wastewater samples from sewersheds tested for SARS-CoV-2 RNA (a method to estimate the number of infected individuals in a sewershed per day) in South Carolina during June-August 2020 estimated the unreported rate for COVID-19 was 12 times (95%CI 6-19) higher than the rate for confirmed…