Technology Development Legacy
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Technology Development Legacy

Over our history the Yeast Resource Center has had a major impact in the development of new technologies that span broad disciplines.  Listed below are some highlights of selected achievements through our history.

Mass Spectrometry

  • Shotgun analysis of protein complexes (10404161).
  • Multidimensional protein identification technology (MudPIT) (11231557).
  • Isotope coded affinity tags (ICAT) (10504701).
  • Stable isotope labeling of vertebrate animals for quantitative analysis (15373428).
  • Post translational modifications in protein complexes (12060738).
  • Characterization of membrane proteins (12692561).
  • Software and methods for the empirical refinement of targeted proteomics assays (20147306).
  • Data-independent acquisition of tandem mass spectra (15782151).
  • Identification of protein-drug interactions using protein stability measurements (20439767).
  • Hardware and approaches for differential ion mobility-mass spectrometry for proteomics (20332067)(18693747).
  • New methods for peptide-centric proteome analysis (26217018).
  • Multiplexed MS/MS for improved data-independent acquisition (23793237).
  • Identification of cross-linked peptides in a complex mixture (25812159).

Yeast Two-Hybrid

  • Genome-wide analyses of protein interactions in yeast (10688190) and Plasmodium falciparum (16267556).
  • First large-scale protein interaction network analysis (11101803).
  • Two-hybrid approach to identify interactions requiring post-translational modification (15208639).
  • Large-scale identification of membrane protein interactions (16093310).

Fluorescence Microscopy

  • A new FRET metric to quantify protein interactions within living cells using a standard wide field fluorescent microscope (15872084).
  • The first Bayesian model for determining protein complex structure using FRET data (25139910).

Protein Structure Prediction

Genome Analysis Technologies

  • Biochemical genomic approach to identify genes by the activity of their products (10550052).
  • High throughput profiling of amino acid levels (20610602).
  • Three-dimensional model of the yeast genome (20436457).
  • Metagenome assembly using Hi-C (24855317).

Mutational Analysis

  • Description of deep mutational scanning of proteins (20711194).
  • Identification of a fundamental protein property by deep mutational scanning (23035249).

Computational Analysis

  • Semi-supervised machine learning for the analysis of proteomics peptide spectrum matches (17952086).
  • Significant speed improvements to proteomics database search engines (21761931).

Biosensors

  • Yeast sensor of ligand binding using stabilization of DHFR (11689849)
  • General sensor strategy using conditional stabilization (26714111)

Protein Design

  • Design of small molecule binding proteins (24005320)
  • Design of a protein based enzyme inhibitor (23827138)
  • Design of protein-binding proteins (24949974)(22634563)

Bibliography