The YRC is a NIGMS Biomedical Technology Research Center based at the University of Washington in Seattle. Click to learn more about us.
Learn more about the technologies being developed by the YRC and how they are being applied to biomedical problems.
Collaborators Since 2011
Annual NIH Dollars Impacted
We are developing technology to produce biosensors based on a ligand-binding domain (LBD) that may, in principle, be applied to any target molecule. The power of this method is illustrated in this paper by development of biosensors for digoxin and progesterone.
In this work, we combine the results of protein cross-linking mass spectrometry with iterative structural modelling to determine the molecular architecture of the 10-member Dam1p protein complex in S. cerevisiae. Using this technique, we can model conformational changes resulting from binding with microtubules.
Amodei, D et al. (2019) Improving Precursor Selectivity in Data-Independent Acquisition Using Overlapping Windows. J. Am. Soc. Mass Spectrom. :. PubMed PMID:30671891
Correia, SP et al. (2019) The circadian E3 ligase complex SCFFBXL3+CRY targets TLK2. Sci Rep 9 (1):198. PubMed PMID:30655559
Sonntag, T et al. (2019) Mitogenic Signals Stimulate the CREB Coactivator CRTC3 through PP2A Recruitment. iScience 11 :134-145. PubMed PMID:30611118
Bao, K et al. (2019) Anti-silencing factor Epe1 associates with SAGA to regulate transcription within heterochromatin. Genes Dev. 33 (1-2):116-126. PubMed PMID:30573453
Keich, U et al. (2018) Controlling the FDR in imperfect matches to an incomplete database. J Am Stat Assoc 113 (523):973-982. PubMed PMID:30546175