The YRC is a NIGMS Biomedical Technology Research Center based at the University of Washington in Seattle. Click to learn more about us.
Learn more about the technologies being developed by the YRC and how they are being applied to biomedical problems.
Collaborators Since 2011
Annual NIH Dollars Impacted
We are developing technology to produce biosensors based on a ligand-binding domain (LBD) that may, in principle, be applied to any target molecule. The power of this method is illustrated in this paper by development of biosensors for digoxin and progesterone.
In this work, we combine the results of protein cross-linking mass spectrometry with iterative structural modelling to determine the molecular architecture of the 10-member Dam1p protein complex in S. cerevisiae. Using this technique, we can model conformational changes resulting from binding with microtubules.
Zimmerman, SG et al. (2017) Proteomics Analysis Identifies Orthologs of Human Chitinase-Like Proteins as Inducers of Tube-Morphogenesis Defects in Drosophila. Genetics :. PMID:28404605
Cao, L et al. (2017) Global site-specific N-glycosylation analysis of HIV envelope glycoprotein. Nat Commun 8 :14954. PMID:28348411
Conkar, D et al. (2017) The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. J. Cell. Sci. 130 (8):1450-1462. PMID:28235840
Matthews, ML et al. (2017) Chemoproteomic profiling and discovery of protein electrophiles in human cells. Nat Chem 9 (3):234-243. PMID:28221344
Thakar, S et al. (2017) Evidence for opposing roles of Celsr3 and Vangl2 in glutamatergic synapse formation. Proc. Natl. Acad. Sci. U.S.A. 114 (4):E610-E618. PMID:28057866