The YRC is a NIGMS Biomedical Technology Research Center based at the University of Washington in Seattle. Click to learn more about us.
Learn more about the technologies being developed by the YRC and how they are being applied to biomedical problems.
Collaborators Since 2011
Annual NIH Dollars Impacted
We are developing technology to produce biosensors based on a ligand-binding domain (LBD) that may, in principle, be applied to any target molecule. The power of this method is illustrated in this paper by development of biosensors for digoxin and progesterone.
In this work, we combine the results of protein cross-linking mass spectrometry with iterative structural modelling to determine the molecular architecture of the 10-member Dam1p protein complex in S. cerevisiae. Using this technique, we can model conformational changes resulting from binding with microtubules.
Matthews, ML et al. (2017) Chemoproteomic profiling and discovery of protein electrophiles in human cells. Nat Chem 9 (3):234-243. PMID:28221344
Thakar, S et al. (2017) Evidence for opposing roles of Celsr3 and Vangl2 in glutamatergic synapse formation. Proc. Natl. Acad. Sci. U.S.A. 114 (4):E610-E618. PMID:28057866
Hickox, AE et al. (2017) Global Analysis of Protein Expression of Inner Ear Hair Cells. J. Neurosci. 37 (5):1320-1339. PMID:28039372
Luhtala, N et al. (2017) Secreted Glioblastoma Nanovesicles Contain Intracellular Signaling Proteins and Active Ras Incorporated in a Farnesylation-dependent Manner. J. Biol. Chem. 292 (2):611-628. PMID:27909058
Timmins-Schiffman, E et al. (2017) Critical decisions in metaproteomics: achieving high confidence protein annotations in a sea of unknowns. ISME J 11 (2):309-314. PMID:27824341