Many of the lessons use the "Urchin Kit" which consists of the following:

• 0.5M potassium chloride solution (3.7grams of KCL in 100ml of distilled water)
• sea water (or Instant Ocean, or artificial sea water)
• fertile sea urchins
• 1-5cc syringe with #25-#30 needle
• plastic or glass pasteur pipets or eyedroppers
• small tubes to store sperm (micro-centrifuge tubes work great) (1-5 milliliters)
• beakers a little smaller in diameter than the diameter of female urchins
• computer to show animations
• microscopes
• Optional - chloramphenicol for long term storage of eggs (30 micrograms/ml) in sterile sea water.
• Optional - video microscopy setup (see Video Microscopy)
• Overhead to show transparencies (see Supplemental Material)

• Urchin Kit
• incubator to store developing embryos at desired temperature (see below)

• Urchin Kit
• sumi ink - an ink used in Asian countries for calligraphy. Available in most art stores.

• Urchin Kit

• Urchin Kit
• incubator to store developing embryos at desired temperature (see below)

• Urchin Kit
• incubator to store developing embryos at desired temperature (see below)
• "Toxins" - students choose, but could be household materials (Clorox, paint, oil, etc)
• Environmental Conditions - heat, light, cold, pH, etc.

• Sea urchin sperm
• Refrigerator
• "Toxins" - students choose, but could be household materials (Clorox, paint, oil, etc)
• Environmental Conditions - heat, light, cold, pH, etc.

• microscopes
• one depression slide with a cover slip or a regular slide with 3 cover slips
• Urchin Kit or other live/prepared material

• microscopes
• prepared slides or living material
• overhead projector
• printout of "figure" from lesson

• The Game Cards -- There is one for sexual and one for asexual. These can be enlarged or projected easily. Print the cards onto a transparency material to use with an overhead.
• The "Genes" - The various phenotypes are represented by a "cast of characters". A character sheet is provided so you can make the many game pieces you will need.
• Scenarios - print these out and use as is or adapt to help make the point you are trying to get across.
• Overhead Projector to either do or demo.

• ability to make empty space in classroom
• overhead or chalkboard
• computer to show animation

• "Classic Coke" - one can per lab group (they will sample it I'm sure)
• ice in a Styrofoam container.
• water to make dilutions with
• graduated cylinder (10 ml will work fine)
• clean test tubes (16x100 work great), 8 for each lab group

• one small package of assorted food colors for each lab group. (Larger size bottles should be purchased for refills for future classes.)
• a light table, students working in groups of 2. (A sheet of translucent white plastic over a sunlit window works great!)
  or
  use the spectrophotometers from the Simple Dilution lesson, students working in groups of 4-5
• set of colored filters (Edmund Scientific #A60,373 $16.75), colored cellophane, or colored transparencies. Make sure they have several colors, so they are forced to make a choice.
• set of 10 clean test tubes of the same size & test tube rack
• set of unknowns made up ahead of time, dilute but still showing visible color. Too high a concentration and the students may think they have solved the problem too easily.
• graduated cylinders (10ml) and eye droppers
• water, paper towels, etc. This lab can be messy!

• See Spectrophotometer in Simple Dilution lesson plan. Use the same blue filter for measuring sperm concentration.
• hemacytometer - Fisher Scientific #02-671-10 $96
• Urchin Kit
• test tubes (to hold 10 ml)
• plastic pipets calibrated to hold 1 ml (E&K Scientific #500025 pkg/1000 ~$18)
• 10 ul Wiretrol - Fisher Scientific #21-175B pkg/100 $15.25

• paper, pencils, overhead projector and overhead
• optional, timer

Look at individual topics. These ideas all interrelate and it would be hard here to give a good list of materials needed.