Result for
Tag: testing
February 9, 2021
Clinical and Economic Impact of Widespread Rapid Testing to Decrease SARS-CoV-2 Transmission
[Pre-print, not peer-reviewed] A transmission model calibrated to the US population suggests that implementing weekly home-based SARS-CoV-2 antigen testing could avert 4 million infections and 19,000 deaths over 60 days while being cost-effective. While a scenario with testing could cost up to $21.5 billion, lower inpatient costs and fewer workdays lost could offset the costs…
February 8, 2021
Implementation of an In-House Real-Time Reverse Transcription-PCR Assay for the Rapid Detection of the SARS-CoV-2 Marseille-4 Variant
[Pre-print, not peer-reviewed] A study evaluating an in-house, real-time RT-PCR (qPCR) assay to detect a SARS-CoV-2 variant with an S477N substitution in the receptor binding domain associated with increased binding affinity to ACE2 found that the assay reliably detected this variant. All 6 cDNA samples from the variant strains tested positive using the assay, whereas…
Field Performance Evaluation of the PanBio Rapid SARS-CoV-2 Antigen Assay in an Epidemic Driven by 501Y.v2 (Lineage B.1.351) in the Eastern Cape South Africa
[Pre-print, not peer-reviewed] A prospective diagnostic evaluation of the field performance of the Abbott PanBio antigen test indicated that the assay reliably detected SARS-CoV-2 variant 501.V2 (lineage B.1.351) infections in patients presenting at mobile community testing centers in South Africa, with sensitivity strongly dependent on viral load (100% detection if cycle threshold (CT) was <20,…
Rapid Electrochemical Detection of Coronavirus SARS-CoV-2Rapid Electrochemical Detection of Coronavirus SARS-CoV-2
A study describing an electrochemical biosensor based on multiplex rolling circle amplification (RCA, in which a short DNA or RNA primer is amplified to make a long single strand) to detect the N and S genes of the SARS-CoV-2 virus showed that the assay could detect as few as 1 copy/microliter of the genes in…
February 5, 2021
Saliva is more sensitive than nasopharyngeal or nasal swabs for diagnosis of asymptomatic and mild COVID-19 infection
Saliva was found to be more sensitive for the detection of SARS-CoV-2 when compared to NP swab or self-administered nasal swab in a cohort of migrant workers in Singapore. Subjects who presented with acute respiratory infection, their asymptomatic roommates, and prior confirmed cases who were undergoing isolation at a community care facility underwent serial RT-PCR…
February 4, 2021
Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR
The DETECTR assay (a combination of RT-LAMP and RT-Cas12-RNP) was found to have similar sensitivity but superior specificity to detect SARS-CoV-2 when compared to qRT-PCR on 378 patient samples derived from routine testing. Since the DETECTR assay is not as susceptible to false negatives from N-gene mutations and not as resource intensive to perform, the…
February 3, 2021
The Limit of Detection Matters: The Case for Benchmarking Severe Acute Respiratory Syndrome Coronavirus 2 Testing
A study modeling the clinical sensitivity of assays to detect SARS-CoV-2 based on their limits of detection (LoD) found that each 10-fold increase in LoD was expected to lower the assay sensitivity by about 13%. Ct values were obtained from 4,700 SARS-CoV-2 positive patients using the Abbott SARS-CoV-2 EUA test. The authors argue that assays…
January 29, 2021
Characteristics and outcomes of clinically diagnosed RT-PCR swab negative COVID-19: a retrospective cohort study
One in five symptomatic patients admitted to the medical department who had a negative SARS-CoV-2 swab received a clinical diagnosis of COVID-19 according to a retrospective cohort study from two large London hospitals. Swab-negative clinical COVID-19 cases were defined as (a) clinical COVID-19 or high level of suspicion as defined by the treating medical team…
A Rapid and Low-Cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-Based RT-qPCR
[Pre-print, not peer-reviewed] Abdel-Sater et al. reported the development of a rapid molecular test to identify the SARS-CoV-2 B.1.1.7 (UK) variant using a set of RT-PCR primers that were designed to confirm the deletion mutations Δ69/Δ70 in the spike and the Δ106/Δ107/Δ108 in the NSP6 gene. The large-scale screening method may help bypass the need…
January 28, 2021
Performance Characteristics of BinaxNOW COVID-19 Antigen Card for Screening Asymptomatic Individuals in a University Setting
The BinaxNow Antigen Card showed low analytical sensitivity (53%) for detecting SARS-CoV-2 infection in a population of asymptomatic or pre-symptomatic individuals when compared to RT-PCR testing for SARS-CoV-2. Okoye et al. (Jan 27, 2021). Performance Characteristics of BinaxNOW COVID-19 Antigen Card for Screening Asymptomatic Individuals in a University Setting. Journal of Clinical Microbiology. https://doi.org/10.1128/JCM.03282-20
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