Yeatman Lab Wiki

Step 1: Create a subject ID folder within the anatomy directory

NLR_###_FL_MR#

For Longitudinal scans, you will want to create a folder for each individual scan, as well as a general directory NLR_###_FL to store an AC-PC aligned image of the total average across scans.<br>

[subid] --seen below-- will include the _MR# for each individual scan.

Data can come off the scanner with arbitrary header information and in parrec format. So for each subject we start by defining a coordinate frame where 0,0,0 is at the anterior commissure, the anterior and posterior commissure are in the same X and Z planes, and the mid-line is centered in the image. Bob Dougherty wrote a nice tool to help with this. See [https://github.com/vistalab/vistasoft/blob/master/mrAnatomy/VolumeUtilities/mrAnatAverageAcpcNifti.m mrAnatAverageAcpcNifti]. The subject's T1-weighted image should be ac-pc aligned, resliced (preserving its resolution), and saved in the subject's anatomy directory.<br> Step 1: In a terminal, convert the PAR/REC files to nifti images

cd /mnt/diskArray/projects/MRI/[subid]
parrec2nii -c -b *.PAR

Step 2: In MATLAB compute the root mean squared (RMS) image and then ac-pc align. If a subject has multiple images then the rms operation should be run on each image and then a cell-array with paths to all the images can be pushed through mrAnatAverageAcpcNifti resulting in a very nice anatomy

T1path = 'Path to t1 weighted image';
T1path = mri_rms(T1path); % Root mean squared image
im = niftiRead(T1path); % Read root mean squared image
voxres = diag(im.qto_xyz)'; % Get the voxel resolution of the image (mm)
mrAnatAverageAcpcNifti({T1path}, '/mnt/diskArray56/projects/anatomy/[subid]/t1_acpc.nii.gz', [], voxres(1:3)')

Freesurfer is a useful tool for segmenting a T1-weighted image and building a cortical mesh. To segment the subject's T1-weighted image using freesurfer from the command line type:

recon-all -i /mnt/diskArray56/projects/anatomy/[subid]/t1_acpc.nii.gz -subjid [subid] -all

Or even better use this handy matlab function written by Jon Winawer to run freesurfer and then also build some useful files that we like to use for data visualization such as a high resolution gray/white segmentation.

fs_autosegmentToITK([subid], '/mnt/diskArray56/projects/anatomy/[subid]/t1_acpc.nii.gz')

For subjects with multiple scans, the output destination should be [subid]_MR[#]. For example, a subject's (205_AB) second scan for the NLR study would be written: NLR_205_AB_MR2. This is in line with the Freesurfer system of notation.