Russell Lab
Division of Hematology, School of Medicine, University of Washington
AAV6 Reagents

 

 

 

 

 

There are several different serotypes of Adeno-Associated Virus (AAV). Type 2 is the original and best-studied serotype. Our laboratory has generated infectious clones of two other serotypes: 3B and 6 (Rutledge et al., 1998). Vectors based on AAV6 have several advantages over conventional AAV2 vectors, including excellent transduction of muscle, lung, liver and other tissues in vivo. AAV vectors can be packaged in serotype 6 capsids by expressing the AAV6 cap genes during vector production. We have generated 2 plasmids that can be used for this purpose. pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production. pDGM6 contains the AAV6 cap genes, AAV2 rep genes, and also adenovirus helper genes (Gregorevic et al., 2004). To obtain these plasmids please fill out and sign the pRepCap6 Materials Transfer Agreement or the pDGM6 Materials Transfer Agreement, and return it to our Technology Transfer Office as instructed on the form. We will then send you the reagents. See specific links for the sequences of pRepCap6 and pDGM6. Please note that in the case of pDGM6, this is a compiled sequence that may contain errors. We use the following diagnostic enzymes to check pDGM6: AatII (10070, 3533, 3327, 2967, and 1945 bp fragments seen) and MfeI (9244, 7695 and 4903 bp fragments). The pRepCap6 sequence is accurate as far as we know. Both plasmids confer ampicillin resistance.

References

  Gregorevic, P., Blankenship, M.J., Allen, J. M., Crawford, R.W., Meuse , L., Miller, D.G., Russell, D.W., and Chamberlain, J.S. (2004). Systemic Delivery of genes to striated muscles using adeno-associated virus vectors. Nat. Med. 10, 828-834.
  Grimm, D., Zhou, S., Nakai, H., Thomas, C. E., Storm, T. A., Fuess, S., Matsushita, T., Allen, J., Surosky, R., Lochrie, M., et al. (2003). Preclinical in vivo evaluation of pseudotyped adeno-associated virus vectors for liver gene therapy. Blood 102, 2412-2419.
  Halbert, C. L., Allen, J. M., and Miller, A. D. (2001). Adeno-associated virus type 6 (AAV6) vectors mediate efficient transduction of airway epithelial cells in mouse lungs compared to that of AAV2 vectors. J Virol 75, 6615-6624.
  Halbert, C. L., Rutledge, E. A., Allen, J. M., Russell, D. W., and Miller, A. D. (2000). Repeat transduction in the mouse lung by using adeno-associated virus vectors with different serotypes. J Virol 74, 1524-1532.
  Rutledge, E. A., Halbert, C. L., and Russell, D. W. (1998). Infectious clones and vectors derived from adeno-associated virus (AAV) serotypes other than AAV type 2. J Virol 72, 309-319.