Small Heat-shock Proteins

sHSPs are a class of diverse chaperone proteins that act as the cell's "first responders" during conditions of high intracellular stress. The ten human sHSPs are refractory to conventional structural techniques. The Klevit lab has used a hybrid approach incorporating solid-state NMR, SAXS, and EM to generate the first atomic-level model of a 24-subunit oligomer of human aB-crystallin.

BRCA1/BARD1 Heterodimer

BRCA1/BARD1, the breast cancer associated protein heterodimer. The Klevit lab is recognized for being the first to solve the solution NMR structure of this medically relevant protein complex.

Ubiquitination Pathway

Protein ubiquitination is involved in the regulation of every cellular process. The modification is carried out by a trio of enzymes known as E1, E2, and E3. In humans, there are over thirty E2s and more than 600 E3s. We are currently studying an array of human E2s and E3s, using approaches that range from structural biology to genetics in model organisms.