(kan) CFP [plasmid pBS5]

Plasmid pBS5 is based upon pyGFP described in Prein et al.. Plasmid pDH22 is coupled with pSH47 to introduce CFP (cyan-fluorescent protein) at the N-terminal end of a protein as described by Prein et al. The mutations described for pDH3 were introduced into pyGFP to convert the GFP to CFP. Some common restriction enzyme sites are shown. The sequence of the forward primer is cggccgccagggg. The sequence of the reverse primer is tttgtacaattcatccataccatggg.

Map:

The DNA sequence of pBS5. The CFP sequence is in blue.

gaacgcggccgccaggggataacttcgtatagcatacattatacgaagttatctagagatctgtttagcttg
cctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgc
gcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcat
ccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaa
acgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatt
tgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccg
aacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctga
tttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaa
gcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggt
cagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgc
atggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtga
aaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaa
cagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattt
tgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcacc
ggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttg
tattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtga
gttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgca
gtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttg
tcatttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatatttttttt
cgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtga
atgctggtcgctatactgctgtcgattcgatactaacgccgccatccagtgtcgaaaacgagctctcgagat
aacttcgtatagcatacattatacgaagttatcagatcatgtctaaaggtgaagaattattcactggtgttg
tcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtg
atgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaacct
tagtcactactttgacttggggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttt
tcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaaga
ccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaag
aagatggtaacattttaggtcacaaattggaatacaactatatttctcacaatgtttacatcactgctgaca
aacaaaagaatggtatcaaagctcatttcaaaattagacacaacattgaagatggttctgttcaattagctg
accattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactc
aatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctg
gtattacccatggtatggatgaattgtacaaaactagtggcctatgcggccgcggatctgccggtctcccta
tagtgagtcgtattaatttcgataagccaggttaacctgcattaatgaatcggccaacgcgcggggagaggc
ggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcga
gcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatg
tgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc
ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatac
caggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc
gcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtc
gttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactat
cgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagta
tttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaa
accaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaa
gatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatg
agattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata
tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctattt
cgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccc
agtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccgga
agggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagct
agagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgc
tcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttg
tgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactc
atggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgag
tactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat
aataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctca
aggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatctttt
actttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgaca
cggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatg
agcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtg
ccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcccttt
cgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgt
ctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctgg
cttaactatgcggcatcagagcagattgtactgagagtgcaccatatggacatattgtcgttagaacgcggc
tacaattaatacataaccttatgtatcatacacatacgatttaggtgacactata

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