FRET - Slide Preparation (Page 1)
To minimize aberrations in the acquired image the yeast must be mounted adjacent to the coverslip. We accomplish this by preparing an agarose pad, depositing the yeast on the pad and then putting down the coverslip. On the next few web pages we describe in detail the preparation of the slide.
We begin by placing two strips of Scotch Tape on either end of a standard microscope slide, such as the pre-cleaned Gold Seal microslides, cat. no. 3010. The slide is cleaned with a kimwipe before the tape is applied.