FRET - Image analysis - FRETR (Page 1)

In the FRET experiment two proteins are labeled, one with CFP and one with YFP. The FRETR value will indicate whether the fluorescent proteins are in close proximity and by implication the two tagged proteins are adjacent, i.e. within 70 angstroms. Image capture and and the initial analysis is the same as described in earlier web pages. A text file is created that has the intensity values for the YFP, YFP background, FRET, FRET background, CFP and CFP background. An example is shown in the next 2 pages.