An Introduction to Mass Spectrometry

Overview

A tandem mass spectrometer is very useful for determining structural information about molecules. The movie depicts the process employed for tandem mass spectrometry of ions. In the first stage, a collection of ions is created in the ion source of the mass spectrometer. The ions are allowed to pass through the first mass analyzer and collision cell and their m/z values are measured in the second mass analyzer. On the basis of data collected in the initial measurement, the first mass spectrometer is set to pass just one m/z value. This ion enters the collision cell and collides with argon. The kinetic energy of ions is converted to vibrational energy and the ions fragment. The m/z values of fragment ions are then determined in the second mass spectrometer. (Movie requires Flash 4™)

An introduction to mass spectrometry is presented here.

Remember that mass spectrometry is not a replacement for good biochemistry, and the old adage "garbage in, garbage out" still applies. The more highly purified the complex the more likely that mass spectrometry will yield useful, biologically relevant information. For now look over the publications to appreciate the approaches that have led to success. Also look over some of the protocols for the preparation of samples.

For more information concerning mass spectrometry proposals you can contact the labs of both Dr. John Yates and Dr. Mike MacCoss. To learn more about submitting proposals, please click here.