Plasmids & Protocols

All requests for plasmids and strains should be submitted through our collaborators' website. Returning collaborators may log in to the left or by clicking here. New collaborators may register with our website here.

• Mass Spectrometry

  An introduction to mass spectrometry.

  Protocol for purification of TAP-tagged proteins.  
  (Click here for printable (PDF) version.)

  Information for submitting samples to the Yates lab.

• Two-hybrid

The Assembly of the 6000 GAL4-AD clones

Primers used for amplifying yeast ORFs and their "common tails"

Vectors: pOBD2 and pOAD

Strains: PJ69-4a and PJ49-4alpha

Recombination Cloning Protocol

• Fluorescence Microscopy

  Tutuorial on our approach to measure FRET between two proteins tagged with CFP and YFP.

  Protocol for Fusing GFP to the Carboxy Terminus of Your Favorite Protein.
  (Click here for printable (PDF) version.)

  We have created a number of different plasmids that are useful for tagging proteins with CFP, YFP, or DsRed. These plasmids can be used for fusions to either the N or C terminus of your protein of interest. We also supply a plasmid to create a positive control for FRET.
  
  

  C-terminal tags (Wach)	N-terminal tags (Prein)	FRET positive control
  Venus, G418 selection (pBS7) (Nagai) YFP, his selection (pDH5) YFP, G418 selection (pDH6) CFP, G418 selection (pDH3) CFP, hygromycin-B selection (pBS4) (Goldstein) DsRed.T1.N1, G418 selection (pTY24) Cerulean, hygromycin-B selection (pBS10) mCherry, G418 selection (pBS34) mCherry, hygromycin-B selection (pBS35)	YFP, G418 selection (pDH22) CFP, G418 selection (pBS5) Venus, G418 selection (pBES2) Cre recombinase (pSH47)	YFP-CFP (his selection) (pDH18) YFP-CFP (G418) [pFA6a-YFP-CFP::KanR]