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Mass Spectrometry
Tandem mass spectrometry is a powerful technique for characterizing a proteome. It allows for studying the structure
and composition of protein complexes, identifying post-translational modification sites, protein expression regulation
and more. Click here to learn more about how the YRC is using mass spectrometry.
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Yeast Two-Hybrid
Dr. Stan Fields' lab has constructed an array of ~6000 yeast transformants,
each designed to express one of the S. cerevisiae open reading frames as a
fusion to the Gal4 activation domain (AD). This array is available as a pooled
library that can be used for genome-wide two-hybrid screens upon generation of
a Gal4 DNA-binding domain (DBD) fusion of the protein of interest.
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Fluorescence Microscopy
Macromolecular complexes can be studied in vivo using color variants of
the green fluorescent protein (GFP) to tag proteins under investigation.
Additionally, fluorescent resonance energy transfer (FRET) can directly
measure protein-protein interactions in vivo. Click here
to learn more about how the YRC is using this technology.
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Protein Structure Prediction
Dr. David Baker's lab has developed techniques for predicting
protein domains (using the GINZU algorithm) as well as 3-D protein
structures based on sequence alone (using the Rosetta algorithm). This technology
helps to elucidate the function of unknown proteins or domains. Click
here to learn more about these technologies.
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Computational Biology
Dr. Bill Noble's lab focuses on the development of machine learning techniques for
application to problems in molecular biology--using Bayesian techniques such as hidden
Markov models, as well as support vector machines and related, non-Bayesian methods.
Click here to learn more about these technologies and how they
are being used by the YRC.
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