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Humoral Immunity Subcore

The Humoral Immunity subcore offers the following services: antibody and antigen testing, recombinant protein and antibody production, viral and cell line culture, and neutralization assays.

Antibody-based Analyses for Antibodies or Antigens
Three antibody-based analyses. Below are protocols and further information on the analyses for which we can provide assistance.

  • Western blot for Envelope proteins
  • HIV-1 Envelope gp120 ELISA for endpoint titer and avidity
  • BioLayer interferometry (Octet)*

*For more information on how to access this service, please contact Noah Sather at noah.sather@seattlechildrens.org

Recombinant Protein and Antibody Production and Purification
Two types of assays are available for recombinant protein research:

  • Expression and Purification of HIV-1 Envelope gp120 and gp140
  • Large-scale purification of IgG from human or macaque plasma

For more information on how to access this service, please contact cfarinfo@uw.edu

Virus and Cell Line Culture and Neutralization Assays
Four types of assays. Below are protocols and further information on the analyses for which we can provide assistance.

  • Isolation of PBMC from PALL filters for subsequent bulk viral infection
  • Bulk Viral Infection of PHA-activated PBMC
  • Pseudovirus production
  • TZM-bl neutralization assay for HIV-1 single round competent viruses

For more information on how to access this service, please contact Noah Sather at noah.sather at cidresearch.org.

Cynthia Derdyn (cderde@uw.edu) and Deborah Fuller (fullerdh@uw.edu)