Dear Colleagues,
We are writing to inform you of recent updates regarding the operations and administration of the UW Keck Imaging Center located in K507. Sharm Knecht will be serving as the acting manager over the coming weeks. During this transitional period, we expect that trained users will continue to have access to the facility and the microscopes they have previously been authorized to operate. We are currently exploring options for onboarding and training new investigators and will share more information as it becomes available. We are optimistic that normal operations at the UW Keck Imaging Center will resume soon. Please direct all questions regarding the Center to uwkeckcenter@uw.edu.
Keck Center Steering Committee
May the FluOResCencE be with you!
The W. M. Keck Microscopy Center provides light microscopy and image analysis services to the University of Washington (UW) research community. External, non-UW users may also use the Keck Center.
The Keck Center operates as a UW recharge center, and is supported by the departments of Physiology and Biophysics (PBio), Pharmacology, Genome Sciences, and Laboratory Medicine & Pathology (DLMP).
The Keck Center was established in 1990 as the W. M. Keck Center for Advanced Studies in Neural Signaling through a grant from the W .M. Keck Foundation so that researchers in the Department of Physiology and Biophysics (PBio) and the Department of Pharmacology could explore how nerve cells acquire, store and transmit information.
Please email the Keck Center at uwkeckcenter@uw.edu for:
Equipment training / reservation calendar access
Equipment demos
Assisted imaging
Consultation: Experimental design, Specimen preparation, Dye selection, etc.
Instrumentation:
Laser-Scanning Confocal Microscopes – Leica SP8X with LIGHTNING, Zeiss 710
Widefield Microscopes – Leica DMI6000, Nikon E600
Environmental Control Equipment
Image Analysis Software – IMARIS (Full License), LASX, FIJI
Capabilities:
Laser-Scanning Confocal Microscopy
3D & 4D (timelapse) scanning, Mosaic tile-scanning*, Multi-point live imaging*, FRAP and FRET imaging*, Resonant scanning*, Sequential scanning up to 5 colors. Spectral scanning, Reflection scanning, LIGHTNING deconvolution for Super-resolution confocal imaging*
*Available on the Leica SP8X only
Multi-color fluorescence imaging, Brightfield, DIC, Phase Contrast, and RGB-color imaging, Z-series, Timelapse imaging, Mosaic tiling, Multi-point live imaging, Extended depth-of-field image processing
FIJI – ImageJ, LASX (Leica Application Suite) with 3D viewer
IMARIS Full License (UWSTF-funded)
Surface and Spot Analysis ,Particle Tracking and Lineage ,Co-localization, Vantage Plotting, XT capability, Cell Analysis, Filament (neuron & vessel) Tracer, Deconvolution Processing, Batch Processing, Animation