May the FluOResCencE be with you!

The W. M. Keck Microscopy Center provides light microscopy and image analysis services to the University of Washington (UW) research community.  External, non-UW users may also use the Keck Center.  The Keck Center operates as a UW cost center, and the Department of Physiology and Biophysics (PBio) and the Department of Pharmacology provide administrative and financial support.

The Keck Center’s IMARIS image analysis license has been FULLY UPGRADED and now includes ALL IMARIS IMAGE ANALYSIS MODULES:
Surface Detection – Spot Detection – Cell Module – Filament (Neuron & Vessels) Tracer – Particle Tracking and Lineage – Co-localization – Deconvolution – Batch Processing – Vantage Plotting – XT module for customized analysis – Animation
This software operates on a brand new, HIGH-POWERED COMPUTER WORKSTATION.  Remote access is possible if coordinated with the Keck Center manager.
This upgrade was funded through UWSTF, and so UW students may now use this amazing and powerful software for FREE.  Non-students may use this software at the Keck Center’s image analysis rate.
LIGHTNING deconvolution (Super-Resolution (SR) confocal imaging) is now possible on the Leica SP8X confocal

Please email the Keck Center manager, Dr. Nathaniel Peters, to schedule:

  • Equipment training / reservation calendar access
  • Equipment demos
  • Assisted imaging
  • Consultation: Experimental design, Specimen preparation, Dye selection, etc.

Keck Center Instrumentation:

Imaging and Image Analysis Capability:

  • Laser-Scanning Confocal Microscopy
    • 3D & 4D (timelapse) scanning
    • Mosaic tile-scanning*
    • Multi-point live imaging*
    • FRAP and FRET imaging*
    • Resonant scanning*
    • Sequential scanning up to 5 colors
    • Spectral scanning
    • Reflection scanning
    • LIGHTNING deconvolution for Super-resolution confocal imaging*
    • *Available on the Leica SP8X only
  • Widefield Microscopy
    • Multi-color fluorescence imaging
    • Brightfield, DIC, Phase Contrast, and RGB-color imaging
    • Z-series
    • Timelapse imaging
    • Mosaic tiling
    • Multi-point live imaging
    • Extended depth-of-field image processing
  • Image Analysis 
    • FIJI – ImageJ
    • LASX (Leica Application Suite)
    • IMARIS Full License (UWSTF-funded)
      • Surface and Spot Analysis
      • Particle Tracking and Lineage
      • Co-localization
      • Vantage Plotting
      • XT capability
      • Cell Analysis
      • Filament (neuron & vessel) Tracer
      • Deconvolution Processing
      • Batch Processing
      • Animation

The Keck Center was established in 1990 as the W. M. Keck Center for Advanced Studies in Neural Signaling through a grant from the W .M. Keck Foundation so that researchers in the Department of Physiology and Biophysics (PBio) and the Department of Pharmacology could explore how nerve cells acquire, store and transmit information.