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Chronology | Project
2011-2012 | 2010-2006 | 2005-2000 | 1999-1995
| Prior to 1995
Publications not available in pdf format may be obtained by contacting
Ray Monnat
Publications 2011-2012
Li H, Ulge UY, Hovde BT, Doyle LA, Monnat RJ Jr. (2012) Comprehensive homing endonuclease target site specificity profiling reveals evolutionary constraints and enables genome engineering applications. Nucleic Acids Res. Mar 1;40(6):2587-98 [ pdf ]
Windbichler, N. Menichelli, M., Papathanos, P.A., Thyme, S.B., Li, H., Ulge, U.Y. Hovde, B.T., Baker, D., Monnat, R.J., Jr., Burt, A. and Crisanti, A. (2011) Genetic drive of a synthetic homing endonuclease-based element in populations of the human malaria vector Anopheles gambiae. Nature May 12;473(7346):212-5. [ pdf ]
Ulge, U.Y., Baker, D.A. and Monnat, R.J., Jr. (2011) Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering. Nucleic Acids Research May;39(10):4330-9. [ pdf ]
Publications 2010
Monnat, R.J., Jr. (2010) Human RECQ helicases: Roles in DNA metabolism, disease pathogenesis and disease therapy. Seminars Cancer Biol. 20(5):329-39. [ pdf ]
Sidorova, J.M. and Monnat, R.J., Jr. (2010) DNA Helicase-deficiency Disorders, in Encyclopedia of Life Sciences, Article # A6065 (in press) [ pdf ]
Mao, F.J., Sidorova, J.M., Lauper, J.M., Emond, M.E. and Monnat, R.J., Jr. (2010) The human WRN and BLM RecQ helicases differentially regulate cell proliferation and survival after chemotherapeutic DNA damage. Cancer Research 70(16):6548-55. [ pdf ] [ supplemental data pdf ]
Thangavel, S., Mendoza-Maldonado, R., Tissino, E., Sidorova, J.M., Yin, J., Wang, W., Monnat, R.J. Jr, Falaschi, A., and Vindigni A. (2010) Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation. Mol Cell Biol. Mar;30(6):1382-9 [ pdf ]
Dhillon, K.K., Sidorova, J.M., Albertson, T.M., Anderson, J.B., Ladiges, W.C., Rabinovitch, P.S., Preston, B.D. and Monnat, R.J., Jr. (2010) Werner syndrome protein loss leads to divergent mouse and human cell phenotypes. DNA Repair (Amst) Jan 2;9(1):11-22 [ pdf ]
Publications 2009
Rey, L., Sidorova, J.M., Puget, N., Boudsocq, F., Biard, D.S.F., Monnat, R.J., Jr., Cazaux, C. and Hoffman, J-S. (2009) Human DNA polymerase eta is required for common fragile site stability during unperturbed DNA replication. Molec Cell Biol Jun;29(12):3344-54 [ pdf ] [ supplemental data pdf ]
Sidorova, J.M., Li, N., Schwartz, D.C., Folch, A. and Monnat, R.J., Jr. (2009) Microfluidic-assisted analysis of replicating DNA molecules. Nature Protocols 4(6):849-61 [ pdf ]
Li, H. Pellenz, S., Ulge, U. Stoddard, B.L. and Monnat, R.J., Jr. (2009) Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins. Nucleic Acids Res. 2009 Jan 19. [ pdf ] [ supplemental data pdf ]
McConnell-Smith, A., Pellenz, S., Davis, L.W., Maizels, N., Monnat, R.J., Jr. and Stoddard, B.L. (2009) Generation of a nicking enzyme that stimulates site-specific targeted gene correction froms the I-AniI homing LAGLIDADG endonuclease. Proc Natl Acad Sci USA Mar 31;106(13):5099-104. [ pdf ] [ supplemental data pdf ]
Publications 2008
Stoddard, B.L. Scharenberg, A.M. and Monnat, R.J., Jr. (2008) Advances in engineering homing endonucleases for gene targeting: Ten years after structures. Chapter 6 in Progress in Gene Therapy 3: Autologous and Cancer Stem Cell Gene Therapy, Eds. R. Bertolotti and K. Ozawa, World Scientific Press, Hackensack, NJ. pp. 135-167. [ pdf ]
Sidorova, J.M. (2008) Roles of the Werner syndrome RecQ helicase in DNA replication. DNA Repair 7:1776-1786 [ pdf ]
Loeb, L.A. and Monnat, R.J., Jr. (2008) DNA polymerases and human disease. Nature Reviews Genetics 9:594-604 [ pdf ]
Berkovich, E., Monnat, R.J., Jr. and Kastan, M.B. (2008) Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites. Nature Protocols 3:915-922. [ pdf ]
Sidorova, J.M., Li, N., Folch, A., Monnat, R.J., Jr. (2008) The RecQ helicase WRN is required for normal replication fork progression after DNA damage or replication fork arrest. Cell Cycle. Jan 4;7(6). [ pdf ]
Publications 2007
Eklund, J.L., Ulge, U.Y., Eastberg, J. and Monnat, R.J., Jr. (2007) Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease. Nucleic Acids Research 35(17):5839-50.[ pdf ]
Sharma, A., Awasthi, S., Harrod, C.K., Matlock, E.F., Khan, S., Xu, L., Chan, S., Yang, H., Thammavaram, C.K., Rasor, R.A., Burns, D.K., Skiest, D.J., Van Lint, C., Girard, A.M., McGee, M., Monnat, R.J. Jr., Harrod, R. (2007) The Werner syndrome helicase is a cofactor for HIV-1 long terminal repeat transactivation and retroviral replication. J Biol Chem. Apr 20;282(16):12048-57. [ pdf ]
Kudlow, B., Kennedy, B.K., and Monnat, R.J. Jr. (2007) Werner and Hutchinson-Gilford progeria syndromes: mechanistic basis of human progeroid diseases. Nature Reviews Molecular and Cellular Biology 8: 394-404. [ pdf ]
Eastberg, J., Eklund, J., Monnat, RJ Jr. and Stoddard, B. (2007) Mutability of an HNH nuclease imidazole general base and exchange of a deprotonation mechanism. Biochemistry 46:7215-7225. [ pdf ]
Monnat, R.J. Jr. (2007) From broken to old: DNA damage, IGF1 endocrine suppression and aging. DNA Repair Sep 1; 6(9): 1386-90. [ pdf ]
Berkovich, E., Monnat, RJ Jr. and Kastan, M. (2007) Roles of ATM and NBS1 in chromatin structure modulation and DNA double strand break repair. Nature Cell Biology 9:683-690. [ pdf ]
Volna, P., Jarjour, J. Baxter, S., Roffler, S., Monnat, RJ Jr., Stoddard, B. and Scharenberg, A. (2007) Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Research 35: 2748-2758. [ pdf ]
Dhillon, K., Sidorova, J., Saintigny, Y., Poot, M., Gollahon, K., Rabinovitch, P.S. and Monnat, R.J. Jr. (2007) Functional role of the Werner syndrome RecQ helicase in human fibroblasts. Aging Cell 6: 53-61. [ pdf ]
Publications 2006
Ashworth, J., Havranek, J.J., Duarte, C.M., Sussman, D., Monnat, R.J., Jr., Stoddard, B.L. and Baker, D. (2006) Computational redesign of endonuclease DNA binding and cleavage specificity. Nature 441:656-659. [ pdf ]
Monnat, R.J., Jr. (2006) 'Werner Syndrome as a Model of Human Aging', In Handbook of Models of Human Aging, P. Michael Conn, ed. Elsevier Academic Press, Chapter 80, pp. 961-976.
Publications 2005-2000
Chevalier, B., Monnat, R.J., Jr. and Stoddard, B.L. (2005) 'The LAGLIDADG Homing Endonuclease Family', in Homing Endonucleases and Inteins, M. Belfort, B.L. Stoddard, D.W. Wood, V. Derbyshire (Eds). Springer, Berlin. pp.33-47.
Awasthi, S., Sharma, A., Wong, K., Zhang, J., Matlock, E.F., Rogers, L., Motloch, P., Takemoto, S., Taguchi, H., Cole, M.D., Luscher, B., Dittrich, O., Tagami, H., Nakatani, Y., McGee, M., Girard, A.M., Gaughan, L., Robson, C.N., Monnat, R.J. Jr., and Harrod, R. (2005) A human T-cell lymphotropic virus type 1 enhancer of Myc transforming potential stabilizes Myc-TIP60 transcriptional interactions. Mol Cell Biol. 25:6178-98. [ pdf ]
Monnat, R.J., Jr. (2005) ‘Werner Syndrome as a Model of Human Aging’, in Handbook of Models of Human Aging, P. Michael Conn, ed. Elsevier Academic Press: chapter 80, pp.961-976.
Chevalier, B.S., Sussman, J., Otis, C., Nöel, A-J., Turmel, M., Lemieux, C., Stephens, K., Monnat, R.J., Jr. and Stoddard, B.L.(2004) Metal-dependent DNA cleavage mechanism of the I-CreI LAGLIDADG homing endonuclease. Biochemistry 43:14015-14022. [ pdf ]
Sussman, D., Chadsey, M., Fauce, S., Engel, A., Bruett, A., Monnat, R. Jr., Stoddard, B. L. and Seligman, L.M. (2004) Isolation and characterization of new homing endonuclease specificities at individual target site positions. J. Mol. Biol. 342:31-41. [ pdf ]
Monnat, R.J., Jr. and Saintigny, Y. (2004) The Werner syndrome protein: in vivo function and disease links. Science’s SAGE-KE (available at: http://sageke.sciencemag.org/cgi/content/full/2004/13/re3). [ pdf ]
Swanson, C., Saintigny, Y., Emond, M.J. and Monnat, R.J., Jr. (2004) The Werner syndrome protein has separable recombination and survival functions. DNA Repair 3:475-482. [ pdf ]
Grandori, C., Wu, K-J., Fernandez, P., Ngouenet, C., Grim, J., Clurman, B.E., Moser, M.J., Oshima, J., Russell, D.W., Swisshelm, K., Frank, S., Amati, B., Dalla-Favera, R. and Monnat, R.J., Jr. (2003) Werner syndrome protein limits MYC-induced cellular senescence. Genes & Development 17:1569-1574.
Chevalier, B.S., Turmel, M., Lemieux, C., Monnat, R.J., Jr. and Stoddard, B.L.(2003) Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI. J. Mol. Biol. 329:253-269. [ pdf ]
Monnat, R.J., Jr. (2002) ‘Werner syndrome’, in WHO/IARC Monograph on Pathology and Genetics of Tumours of Soft Tissue and Bone, edited by C. Fletcher, K. Unni and F.Mertens. IARC Press, Lyon. pp. 366-367.
Chevalier, B.S., Kortemme, T., Chadsey, M.S., Baker, D., Monnat, R.J., Jr. and Stoddard, B.L. (2002) Design, activity and structure of E-DreI, a highly site-specific artificial endonuclease Molecular Cell 10:895-905. [ pdf ]
Saintigny, Y., Makienko, K., Swanson, C., Emond, M.J. and Monnat, R.J., Jr. (2002) A homologous recombination resolution defect in Werner syndrome Molec. Cell. Biol. 22:6971-6978. [ pdf ]
Colgin, L.M., Hackmann, A.F.M., Emond, M.J. and Monnat, R.J., Jr. (2002) The unexpected landscape of in vivo somatic mutation in a human epithelial cell lineage Proc. Natl. Acad. Sci. USA 99:1437-1442. [ pdf ]
Saintigny, Y. and Monnat, R.J., Jr. (2002) L’Hélicase RecQ, dont les altérations sont à l’origine du syndrome de Werner, participe à la réparation de l’ADN par recombinaison homologue. Médecine Sciences 18:79-85.
Monnat, R.J., Jr. (2001) Cancer pathogenesis in the human RecQ helicase deficiency syndromes, in ‘From Premature Gray Hair to Helicase: Werner Syndrome Implications for Aging and Cancer’ , edited by M. Goto and R.W. Miller. GANN Monograph on Cancer Research 49: 83-94.
Prince, P.R., Emond, M.J. and Monnat, R.J., Jr. (2001) Loss of Werner syndrome protein function promotes aberrant mitotic recombination Genes & Development 15:933-938. [ pdf ]
Chevalier, B.S., Monnat, R.J., Jr. and Stoddard, B.L. (2001) The LAGLIDADG homing endonuclease I-CreI shares three metals between two active sites. Nature Structural Biology 8:312-316. [ pdf ]
Galburt, E.A., Chadsey, M.S., Jurica, M.S., Chevalier, B.S., Ehro, D., Tang, W., Monnat, R.J., Jr. and Stoddard, B.L. (2000) Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site. J. Mol. Biol. 300:877-887. [ pdf ]
Moser, M.J., Bigbee, W.L., Grant, S.G., Emond, M.J., Langlois, R.G., Jensen, R.H., Oshima, J. and Monnat, R.J., Jr. (2000) Genetic instability and hematologic disease risk in Werner syndrome patients and heterozygotes. Cancer Research 60:2492-2496. [ pdf ]
Publications 1999-1995
Galburt, E., Chevalier, B., Tang, W., Jurica, M.S., Flick, K.E., Monnat, R.J., Jr. and Stoddard, B.L. (1999) A novel endonuclease mechanism directly visualized for I-PpoI. Nature Structural Biology 6:1096-1099. [ pdf ]
Monnat, R.J., Jr. (1999) Unusual features of thyroid carcinomas in Japanese patients with Werner syndrome and possible genotype-phenotype relations to cell type and race (originally entitled ‘No association of specific WRN mutations with thyroid cancer risk or histologic subtype in Werner syndrome patients’ prior to editorial retitling). Cancer 86:728-729. [ pdf ]
Prince, P.R., Ogburn, C.E., Moser, M.J., Emond, M.J., Martin, G.M. and Monnat, R.J., Jr. (1999) Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines. Human Genetics 105:132-138. [ pdf ]
Colgin, L.M., Hackmann, A.F.M., and Monnat, R.J., Jr. (1999) Different somatic and germline HPRT1 mutations promote use of a common, cryptic intron 1 splice site. Human Mutation 13:504-505. [ pdf ]
Monnat, R.J., Jr., Hackmann, A.F.M. and Cantrell, M.A. (1999) Generation of highly site-specific DNA double strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI. Biochem. Biophys. Res. Comm. 255:88-93. [ pdf ]
Bennett, S.E., Umar, A., Kodama, S., Barrett, J.C., Monnat, R.J., Jr. and Kunkel, T.A. (1999) Evidence against a role for the Werner syndrome gene product in DNA mismatch repair. Molecular Biology of Aging, Alfred Benzon Symposium Proceedings, No. 44. Bohr, V.A., Clark, B.F.C. and Stevnsner, T. Munksgaard, Copenhagen. pp.214-224.
Jurica, M.S., Monnat, R.J., Jr. and Stoddard, B.L. (1998) DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI. Molecular Cell 2:469-476. [ pdf ]
Flick, K.E., Jurica, M.S., Monnat, R.J., Jr. and Stoddard, B.L. (1998) DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI. Nature 394:96-101. [ pdf ]
Argast, G., Stephens, K.M., Emond, M.J., and Monnat, R.J., Jr. (1998) I-PpoI and I-CreI homing site sequence degeneracy determined by random mutagenesis and sequential in vitro enrichment. J. Mol. Biol. 280:345-353. [ pdf ]
Flick, K.E., McHugh, D., Heath, J.D., Stephens, K.M., Monnat, R.J., Jr., and Stoddard, B.L. (1997) Crystallization and preliminary X-ray studies of I-PpoI: a nuclear, intron-encoded homing endonuclease from P. polycephalum. Protein Science 6:1-4. [ pdf ]
Seligman, L.M., Stephens, K.M. and Monnat, R.J., Jr. (1997) Genetic analysis of the Chlamydomonas reinhardtii I-CreI mobile intron homing system in Escherichia coli. Genetics 147:1653-1664. [ pdf ]
Bennett, S., Umar, A., Oshima, J., Monnat, R.J., Jr. and Kunkel, T.M. (1997) Mismatch repair in extracts from Werner syndrome cell lines. Cancer Research 57: 256-2960.
Heath, P.D., Stephens, K.M., Monnat, R.J., Jr. and Stoddard, B.M. (1997) The structure of I-CreI: a Group I intron-encoded homing endonuclease. Nature Structural Biology 4:468-476.
Stephens, K.M., Monnat, R.J., Jr., Heath, P.D., and Stoddard, B.M. (1997) Crystallization and preliminary X-ray analysis of the Chlamydomonas reinhardtii I-CreI mobile intron endonuclease. Proteins: Structure, Function and Genetics 28:137-139. [ pdf ]
Brooks-Wilson, A.R., Emond, M. and Monnat, R.J., Jr. (1997) Paradoxically low loss of hetero-zygosity (LOH) in genetically unstable Werner syndrome cell lines. Genes, Chromosomes & Cancer 18:133-142. [ pdf ]
Martin, G.M., Ogburn, C.E., Colgin, L.M., Gown, A.M., Edland, S.D. and Monnat, R.J., Jr. (1996) Somatic mutations are frequent and increase with age in human kidney epithelial cells. Human Molec. Genetics 5:215-221. [ pdf ]
Publications prior to 1995
Monnat, R.J., Jr. (1992) Werner syndrome: molecular genetics and mechanistic hypotheses. Exp. Gerontol. 27:447-453. (first prediction that that the WRN protein may have helicase activity)
Monnat, R.J., Jr., Chiaverotti, T.A., Hackmann, A.F.M., and Maresh, G.A. (1992) Molecular structure and genetic stability of human hypoxanthine phosphoribosyltransferase (HPRT) gene duplications. Genomics 11:788-796.
Monnat, R.J., Jr., Hackmann, A.F.M., and Chiaverotti, T.A. (1992) Nucleotide sequence analysis of human hypoxanthine phosphoribosyltransferase (HPRT) gene deletions. Genomics 11:777-787.
Chiaverotti, T.A., Battula, N. and Monnat, R.J., Jr. (1991) Rat hypoxanthine phosphoribosyl-transferase cDNA cloning and sequence analysis. Adv. Exp. Biol. Med. 309B:117-120.
Fukuchi, K.-i., Tanaka, K., Kumahara, Y., Marumo, K., Pride, M.B., Martin, G.M., and Monnat,R.J., Jr. (1990) Increased frequency of 6-thioguanine-resistant peripheral blood lymphocytes in Werner syndrome patients. Human Genetics 84:249-252.
Fukuchi, K.-i., Martin, G.M., and Monnat, R.J., Jr. (1989) Mutator phenotype of Werner syndrome is characterized by extensive deletions. Proc. Natl. Acad. Sci. USA 86:5893-5897. (errata in Proc. Natl. Acad. Sci. USA 86:7994, 1989). [ pdf ]