Immunology Core

About

The Immunology Core provides advanced tools and training for evaluating humoral and cellular responses to HIV infection.

The assays, reagents, technology, and education offered by the Core support CFAR research involved with the prevention, detection, and treatment of HIV infection and AIDS. Most Core services are used by investigators to help quantify immune responses in plasma, serum, secretions, or cells from HIV-infected patients or lentivirus-infected nonhuman primates. The types and levels of support available from Core faculty are flexible—from quick telephone or email troubleshooting consultations to intensive group trainings on specific technologies; from simple cytometry experiments using one to three colors to cutting-edge experiments using up to 18 colors; and from rapid immune analysis of a few samples for a pilot assessment or hypothesis generation to longer-term collaborations involving joint study design and execution. Services are accessible through our three subcores:

Cellular Immunity and Flow Cytometry Subcore: Experiment Design, Consultations, Laboratory Support; Flow cytometry (cell sorting, Te cell response, T/B cell phenotyping, special studies); specialized assays of cell function/activity, proliferation, or phenotyping (NK, Treg, DC); cytokine bead array; viral suppression assays.

Humoral Immunity Subcore: Antibody and antigen testing; recombinant protein and antibody production; viral and cell line culture; neutralization assays.

Mucosal Immunity and Non-Human Primate Immunology Subcore: Advice in study design and testing of vaccines and therapies in SIV and SHIV monkey models for AIDS and analysis of humoral, cellular, and innate immune responses in the blood and mucosal compartments of nonhuman primates.

Single-Cell Quantitative RNAseq and High Content Data Analysis Subcore: Whole cell transcript profiling, high-throughput targeted RNA assays, quantitative profiling of circulating proteins and antibodies, TCR and BCR sequencing, analysis of multiple data types (DNA, RNA, proteomic, flow cytometry).

Services
People

Stephen De Rosa, MD
Core Director; Cellular Immunity and Flow Cytometry Subcore Director
Email: sderosa at fredhutch.org
Telephone: 206-667-1681

Ana Gervassi, PhD
Core Associate Director; Cellular Immunity and Flow Cytometry Immunity Subcore Director
Email: ana.gervassi at seattlebiomed.org
Telephone: 206-256-7198

Noah Sather, PhD
Humoral Immunity Subcore Director
Email: noah.sather at seattlechildrens.org

Deborah Fuller, PhD
Non-Human Primate Immunology Subcore Director
Email: fullerdh at wanprc.org

Florian Hladik, PhD
Mucosal Immunity and Non-Human Primate Immunology Subcore Director
Email: fhladik at fredhutch.org

Michael Gale, PhD
Single-Cell Quantitative RNAseq and High Content Data Analysis Subcore Director
Email: mgale at uw.edu

Raphael Gottardo, PhD
Single-Cell Quantitative RNAseq and High Content Data Analysis Subcore Director
Email: rgottard at fredhutch.org