Laser-scanning Confocal Microscopes
The Keck Center’s laser-scanning confocal microscopes use laser excitation, motorized scanning mirrors, confocal pinholes, and amplification or photon-counting detectors to collect high-resolution optical sections that exclude out-of-focus light. Through serial, optical sectioning of fluorescently-labelled samples, these systems are capable of producing high-resolution 3D and 4D datasets.
Leica SP8X Confocal
All publications that include data acquired on the Leica SP8X confocal must acknowledge NIH grant S10 OD016240.
- Leica DMI6000 microscope
- Motorized XY stage capable of mosaic tiling and multi-point imaging
- Motorized Z-galvo stage for rapid Z movement
- 10X and 20X dry objectives
- 40X and 63X oil immersion objectives
- 25X and 63X water immersion objectives
- Adaptive focus control (AFC) for maintaining focus during live imaging and/or mosaic tiling
- 470-670nm tunable White Light Laser (WLL) system
- 405, 458, 476, 488, 496, and 514nm fixed wavelength laser lines
- 3 PMT amplification detectors
- 2 photon-counting HyD detectors with lifetime gating
- Trans-PMT detector for DIC imaging
- Resonant scanner for rapid, live-imaging
- STP8000 backlit touchpad (funded by UWSTF)
- KLONDIKE scan head electronics (funded by UWSTF)
- Expert CUDA Workstation (funded by UWSTF)
- Leica LASX Expert software (funded by UWSTF)
- Navigator
- FRAP
- FRET
- Lightning Deconvolution (funded by UWSTF)
KLONDIKE scan head electronics, CUDA computer workstation, and LASX Expert software with Lightning deconvolution were funded by the UW Student Technology Fee.
![](https://depts.washington.edu/keck/wordpress/wp-content/uploads/2019/10/Leica-SP8X-Confocal-1024x768.jpg)
![](https://depts.washington.edu/keck/wordpress/wp-content/uploads/2019/10/Leica-SP8X-Confocal-2-1024x768.jpg)
Zeiss LSM 710 Confocal
- Zeiss Axio Observer Z.1 microscope
- Manual stage with slide, dish, and plate inserts
- 5X, 10X and 20X dry objectives
- 40X, 63X, and 100X(no UV) oil immersion objectives
- 40X and 63X water immersion objectives
- 405, 458, 488, 514, 561, 594, and 633nm fixed wavelength laser lines
- 2 PMT amplification detectors
- 1 spectral PMT amplification detector
- Tunable emission for all detectors
- Trans-PMT detector for DIC imaging
- Zeiss Zen 2009 software
![](https://depts.washington.edu/keck/wordpress/wp-content/uploads/2019/10/Zeiss-710-Confocal-1024x768.jpg)