ResourceDescriptionCystic FibrosisControls w/o CF# of samples/ sectionsStart DateContactRelated Grants/Studies
Airway and Lung ParenchymaTissue from explanted lungs from patients with CF; typically processed within 24 -48 hours post-surgery332009Moira Aitken, MD
Pradeep Singh, MD
Singh, CFF SINGH11GO
Pancreatic sectionsPancreatic autopsy tissue sections from patients with cystic fibrosis, with and without CF related diabetes, and non-CF controls with and without Type II diabetes435525 sections per participant, 1075 CF; 1375 non-CF2012Rebecca Hull, PhDHull,
P30 DK089507 -Pilot
StoolStool samples collected longitudinally over 12 month period from infants and young children; refrigerated after collection; frozen at -80 C within 24 – 72 hours15131402011Luke Hoffman, MD, PhDHoffman,
P30 DK089507 - Pilot
Saliva
Stimulated and unstimulated saliva collected and frozen at -80 C within 24 hours after collection.30602014Donald Chi, DDS, PhDChi, CFF R565-CR11 – Pilot
Expectorated SputumSpontaneously expectorated sputum collected longitudinally for 3 observational trials; refrigerated at 4 C after collection and stored at -80 C within 24 – 48 hours after collection451202011Luke Hoffman, MD, PhDHoffman, CFF – HOFFMA11A0
Expectorated SputumSpontaneously expectorated sputum collected longitudinally for 3 observational trials; refrigerated at 4 C after collection and stored at -80 C within 24 – 48 hours after collection545872009Pradeep Singh, MD
Singh,
K24 HL102246
R01 HL110879
R01 AI101307
Expectorated SputumSpontaneously expectorated sputum collected longitudinally for 3 observational trials; refrigerated at 4 C after collection and stored at -80 C within 24 – 48 hours after collection12412013Pradeep Singh, MD
Singh,
R01 AI101307
Microbial DNA prepsMicrobial DNA preps available from explanted lungs81152009Pradeep Singh, MD
Singh,
K24 HL102246,
CFF SINGH11GO,
Bacterial IsolatesBacterial populations isolated from expectorated sputum samples (96 isolates per “population”)54406 bacterial populations including 27 from patients with exacerbation; 144 from patients during antibiotic treatment2009Pradeep Singh, MD
Singh,
K24 HL102246
R01 HL110879,
R01 AI101307
Bacterial isolatesPopulations of bacterial isolates cultured from explanted lung tissue of upper, middle and lower lobes and lingula13131 populations from 10 lungs2009Pradeep Singh, MD
Singh, CFF SINGH11GO
Bacterial IsolatesPopulations of P. aeruginosa isolates from 8 CF subjects with the G551D genotype prior to (day 0) and after (days 2, 8, 90, 210, 420) receiving ivacaftor 841 populations of 96 isolates each from 8 subjects2013Pradeep Singh, MD
SerumSamples banked at multiple time points during oral glucose tolerance testing; frozen at -80 C11662014Ron Gibson, MD, PhDMotaghedi, SCRI Academic Excellence Fund
Historical Biospecimens
Bacterial IsolatesLongitudinal clinical bacterial isolates (P. aeruginosa, S. aureus [MSSA, MRSA, SCV], S maltophilia, Achromobacter spp., B. cepacia complex), cultured from upper and lower respiratory specimens and explanted lung tissue from individual patients cared for at SCH and UWMC; sources detailed in Micro Core and described on the CFRTC websiteAll isolates from individuals with CF
Over 30,000 clinical isolates
Jane Burns, MD
Bacterial IsolatesIsolates of P. aeruginosa, S. aureus [MSSA, MRSA, SCV], S maltophilia, Achromobacter spp., and B. cepacia complex derived from 14 observational and interventional studies processed in the TDN Center for CF Microbiology at Seattle Children’s; specific studies detailed in Micro Core Table 1 and described on CFRTC website.All isolates from individuals with CF9500 isolates from 14 clinical trialsJane Burns, MDMicro core isolates
derived from 14 clinical trials
Ordered transposon mutant library of Pseudomonas aeruginosaCreated a near-saturation sequence defined transposon mutant library in which individual mutants are arrayed and can be retrieved for distribution. 'Random' transposon insertion mutants were arrayed and sequenced to define the transposon insertion locations in the genome of P. aeruginosa strain PA01.9437Kiara Held
SerumLongitudinal serum samples collected pre, during post therapeutic intervention in a multicenter clinical trial in pediatric patients661715 aliquotsRon Gibson, MD, PhDRamsey and Gibson, IT 004 Clinical Trial
Bronchoalveolar lavage (BAL)BAL collected pre- and post- therapeutic intervention in a multicenter clinical trial in pediatric patients441215 aliquotsRon Gibson, MD, PhDRamsey and Gibson, IT 004 Clinical Trial
Data Repository
Research clinical dataData from 21 Phase 3 multi-center trials involving over 3000 patients; described on the CFRTC website> 3000TDN contact:
Sonya Heltshe