ResourceDescriptionCystic FibrosisControls w/o CF# of samples/ sectionsStart DateContact
Airway and Lung ParenchymaTissue from explanted lungs from patients with CF; typically processed within 24 -48 hours post-surgery332009Pradeep Singh, MD
Pancreatic sectionsPancreatic autopsy tissue sections from patients with cystic fibrosis, with and without CF related diabetes, and non-CF controls with and without Type II diabetes43553-10 sections/participant, ~300 CF; ~400 non-CF2012Chuck Frevert, DVM, PhD
StoolStool samples collected longitudinally over 12 month period from infants and young children; refrigerated after collection; frozen at -80 C within 24 – 72 hours15131402011Luke Hoffman, MD, PhD
Expectorated SputumSpontaneously expectorated sputum collected longitudinally; refrigerated at 4 C after collection and stored at -80 C within 24 – 48 hours after collection451202011Luke Hoffman, MD, PhD
Expectorated SputumSpontaneously expectorated sputum collected longitudinally; refrigerated at 4 C after collection and stored at -80 C within 24 – 48 hours after collection545872009Pradeep Singh, MD
Expectorated SputumSpontaneously expectorated sputum collected longitudinally; refrigerated at 4 C after collection and stored at -80 C within 24 – 48 hours after collection12412013Pradeep Singh, MD
Microbial DNA prepsMicrobial DNA preps available from explanted lungs161232009Pradeep Singh, MD

Luke Hoffman, MD, PhD
Bacterial IsolatesBacterial populations isolated from expectorated sputum samples (96 isolates per “population”)54406 bacterial populations including 27 from patients with exacerbation; 144 from patients during antibiotic treatment2009Pradeep Singh, MD
Bacterial isolatesPopulations of bacterial isolates cultured from explanted lung tissue of upper, middle and lower lobes and lingula13131 populations from 10 lungs2009Pradeep Singh, MD
Bacterial IsolatesPopulations of P. aeruginosa isolates from 8 CF subjects with the G551D genotype prior to (day 0) and after (days 2, 8, 90, 210, 420) receiving ivacaftor 841 populations of 96 isolates each from 8 subjects2013Pradeep Singh, MD
SerumSamples banked at multiple time points during oral glucose tolerance testing; frozen at -80 C11662014Ron Gibson, MD, PhD
Airway and Lung ParenchymaFormalin fixed paraffin embedded (FFPE) tissue sections from lung biopsies, lobectomies and postmortem lungs from patients with CF; subset have lung snap frozen stored at -80C. Age-matched controls 4325Multiple sections per participant. 10 stored frozen 1975Gail Deutsch, MD
PancreasFormalin fixed paraffin embedded (FFPE) tissue sections from autopsies of patients with cystic fibrosis, with and without CF related diabetes, and age-matched non-CF controls; subset have pancreas snap frozen stored at -80C2016Multiple sections per participant 3 stored frozen1975Gail Deutsch, MD
LiverFormalin fixed paraffin embedded (FFPE) tissue sections from liver biopsies from patients with CF processed within 24 hours post-surgery; subset have liver snap frozen stored at -80C15TBD3-10 sections per participant 12 stored frozen2002Gail Deutsch, MD
Bowel resections for meconium ileusFormalin fixed paraffin embedded (FFPE) tissue sections from bowel resections from patients with CF processed within 24 hours post-surgery11TBDMultiple sections per participant2002Gail Deutsch, MD
Gastrointestinal biopsiesFormalin fixed paraffin embedded (FFPE) tissue sections from gastrointestinal biopsies from patients with CF processed within 24 hours post-surgery47TBD3-10 sections per participant2002Gail Deutsch, MD
Historical Biospecimens
Bacterial IsolatesLongitudinal clinical bacterial isolates (P. aeruginosa, S. aureus [MSSA, MRSA, SCV], S maltophilia, Achromobacter spp., B. cepacia complex), cultured from upper and lower respiratory specimens and explanted lung tissue from individual patients cared for at SCH and UWMC.All isolates from individuals with CF
Over 25,000 clinical isolates
Luke Hoffman, MD, PhD

MOAC request form
Bacterial IsolatesIsolates of P. aeruginosa, S. aureus [MSSA, MRSA, SCV], S maltophilia, Achromobacter spp., and B. cepacia complex derived from 14 observational and interventional studies processed in the CFF Center for CF Microbiology at Seattle Children's; specific source studies.All isolates from individuals with CF9500 isolates from 14 clinical trialsLuke Hoffman, MD, PhD

MOAC request form
Ordered transposon mutant library of Pseudomonas aeruginosaCreated a near-saturation sequence defined transposon mutant library in which individual mutants are arrayed and can be retrieved for distribution. 'Random' transposon insertion mutants were arrayed and sequenced to define the transposon insertion locations in the genome of P. aeruginosa strain PA01.9437Steve Salipante

Pa Mutant Library
SerumLongitudinal serum samples collected pre, during post therapeutic intervention in a multicenter clinical trial in pediatric patients661715 aliquotsRon Gibson, MD, PhD
Bronchoalveolar lavage (BAL)BAL collected pre- and post- therapeutic intervention in a multicenter clinical trial in pediatric patients441215 aliquotsRon Gibson, MD, PhD
Data Repository
Research clinical dataData from 21 Phase 3 multi-center trials involving over 3000 patients; described on the CFRTC website> 3000TDN contact:
Sonya Heltshe

Powered by WordPress