CF Research Translation Center and Research Development Program

University of Washington
UW Health Sciences, K-140
Genome Sciences, Box 357710
Seattle, WA 98195


The purpose of the Genomics Core is to provide instrumentation, computational infrastructure, technical and analytic expertise, and guidance in order to broadly enable and to enhance the use of genomic analysis in Cystic Fibrosis (CF) research. Among other areas of research focus, the Genomics Core places a special emphasis in understanding host-microbe interactions in individuals with CF. The Aims of the Core are to: 1) Provide sequencing and computational resources to study microbial communities in the CF gut and airways, 2) Provide cutting-edge sequencing and computational resources that advance research on pathogens associated with CF disease states, 3) Provide advanced services to understand gene expression and molecular physiology of human cells that are relevant to CF disease states, and 4) Develop novel technologies for the genome-scale analysis of CF microbiology. The Core will play a primary role in driving projects to define how the microbiomes of the gastrointestinal (GI) and respiratory tracts change across the full lifespan of patients with CF, and to understand how the administration of highly effective CFTR modulators changes the composition and metabolic functions of the microbiome and its interface with host cells. The primary services of the core are:

  • Consultation on genomic methods and experimental design.
  • Data analysis, data management and data sharing.
  • DNA sequencing of bacterial pathogens for whole genome assembly and genetic variant detection.
  • Metagenomic and 16s rRNA Microbiome analysis, data generation and analysis.
  • Transcriptome sequencing and analysis (RNA-Seq) and targeted gene expression analysis (qRT-PCR).
  • Gut microbe quantitation by Digital Droplet PCR (dPCR).
  • Genome-scale genetic screens using transposon mutant sequencing (Tn-Seq).
  • New sequencing technology and innovations.

Supported by a grant from the National Institutes of Health (NIDDK P30 DK 089507) and the Cystic Fibrosis Foundation (SINGH19R0). Please reference these grant numbers on all publications resulting from support provided by this Core Center grant.