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ADRC Project 3
NCSF Proteome of Geriatric Dementias
Thomas Montine, M.D., Ph.D., Principal Investigator
tmontine@u.washington.edu

This project will use resources of the Clinical Core and Neuropathology Core to test the hypothesis that quantification of the CSF proteome can be used to identify a roster of proteins that objectively characterizes the different facets of geriatric dementia. Aims are:

1. Discovery - The project will use isotope coated affinity tagging (ICAT) coupled with 2-dimensional liquid chromatography/electrospray ionization mass spectrometry (2-DLC/ESI-MS) to determine relative quantitative differences in the CSF proteome among geriatric dementias and normal aging;
2. Confirmation - The project will use immunochemical methods to confirm major findings of (1) and develop a multi-analytic profile for geriatric dementias; 3) Validation - Findings will be validated in lumbar CSF from a separate set of controls, Mild Cognitive Impairment, and probable AD or DLB patients from UW and OHSU Clinical Cores; and 4) Data and Sample Exchange - Analysis results will be posted on the UW Neuropathology website and linked to the NACC. Aliquots of CSF analyzed will be available to others for alternative proteomic or other discovery based methods in investigation of geriatric dementia biomarkers.

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UW ADRC Director--Murray Raskind, M.D.
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Last Modified: Friday, 09-Jan-2009 13:31:35 PST