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SetupPrimePump

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OPEN CONNECTION TO CONTROLLER

1. open putty (or the arduino serial monitor)
   • select serial under "Connection type" (skip for arduino)
   • select the com port that is attached to the controller (eg "COM7") under "Serial Line" (tools->serial port for arduino)
   • (select tools->serial monitor in the arduino software)
   • Enter 19200 under "Speed" (19200 baud in arduino software)
   • click "Open" (skip for arduino)
2. you should see a stream of numbmers arrive approximately every 3 seconds, each number should be >100,000 if not something is wrong.

PRIME SIPHON

1. Close pinch valves with the controller program
2. Unclamp media line originating from media filled carboy
3. Remove air from the media line (tapping it works best)

FILL CHAMBERS WITH MEDIA

1. One by one, open the valve for each chamber using the controller program
2. Use the media solenoid valve manual override to add media to each tube until the level reaches the bottom of the effluent glass capillary
   1. At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)

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