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Setup

Hookup Overview

Hookup overview ( PDF version).

Setup Overview

Day -1:

1. Prepare components
   • Prepare Media (typically in aspirator bottle)
   • Assemble closed system and autoclave

Day 0:

1. Attach non-autoclavable parts
   • Attach sterile syringe
   • Attach media line to media bottle
2. Attach closed system to machinery
   • Attach syringe to syringe pump
   • Place culture vessels into their chambers
   • Attach media tubing to pinch valves
   • Connect air tubing to pressure regulators
   • Connect effluent tubing to waste bottles
3. unclip/open media bottle tube
4. Prime pump
   • Prime siphon
   • Fill chambers with media
5. Start software and blank
6. Innoculate